Gene: pha-4 - Caenorhabditis elegans(see also ageing factor AF_008989 *)
Last modified: Dec 09, 2014. Release 1 • Page created: May 19, 2024
Summary
Gene Symbol | : | pha-4 | ||||||||||||||||||||||||||||
NCBI Gene ID | : | 180357 * | ||||||||||||||||||||||||||||
Species | : | Caenorhabditis elegans | ||||||||||||||||||||||||||||
Gene Synonyms | : | Ce-fkh-1 | CE15998 | CE40228 | CE40229 | CELE_F38A6.1 | F38A6.1 | F38A6.1a | F38A6.1b | F38A6.1c | fkh-1 | pha-4 | Protein PHA-4 | WP:CE15998 | WP:CE40228 | WP:CE40229 Source: GPSDB |
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Gene Homologs | : | |||||||||||||||||||||||||||||
Ageing Relevance Analysis | : |
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Ageing Factor Stable ID | : | AF_005316 | ||||||||||||||||||||||||||||
Download | : | XML |
*
AgeFactDB contains further genes associated with gene symbol pha-4 and the species Caenorhabditis elegans: AF_008989
Protein Information
Protein Name | Species | UniProt Accession Number | UniProt Entry Name | Protein Function | Sequence |
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Defective pharyngeal development protein 4 | Caenorhabditis elegans | Q17381 (UniProtKB/Swiss-Prot) | PHA4_CAEEL (UniProtKB/Swiss-Prot) | Acts as a transcription factor required for formation of the pharyngeal primordium. Binds to the consensus sequence 5'-T[AG]TT[TG][AG][TC]-3' with variations of the sequence affecting onset of target gene expression. Activates a wide array of pharyngeal genes including ceh-22 and myo-2 and represses ectodermal genes lin-26 and elt-3, ensuring pharyngeal cell fate. Required for recruitment of htz-1 to a subset of pharyngeal promoters to ensure gene activation and also acts synergistically with tbx-2 in pharyngeal development. Acts in the regulation of diet-restriction-mediated longevity by increasing expression of sod-1, sod-2, sod-4 and sod-5 but not sod-3. | View |
Observations
Ageing Phenotype
Data Type 1
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
# | Observation Stable ID | Species | Gene | Other Ageing Factor | Description | PubMed | Source | ||||||
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1 | OB_005714 | Caenorhabditis elegans |
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PHA-4 may play a role in the life-extending effects of dietary restriction. RNA interference decreased lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains. Overexpression slightly increased longevity. | 17476212 | GenAge | |||||||
2 | OB_008022 | Caenorhabditis elegans |
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pha-4 is required for multiple forms of DR. RNAi of pha-4 completely cancels out the lifespan extension of eat-2 mutation. Mutants of pha-4 do not respond to bacterial DR. Therefore, loss of pha-4 completely blocks the response to varying food concentration. Moreover, pha-4 expression is increased in response to DR in wild-type. pha-4 overexpression increases longevity of wild-type only slightly, but significant that of daf-16 mutants. The response to DR involves the PHA-4-dependent expression of sod-1, sod-2 and sod-5. Reduction of pha-4 does not suppress the long lifespan of daf-2 mutants or animals with defective electron transport chain [ 17476212]. IF significantly extends lifespan of pha-4 [ 19079239]. sDR extends lifespan of mutants with a temperature sensitive allele of pha-4 or pha-4 RNAi knockdown, but not daf-16 RNAi [ 19239417]. PHA-4 may play a role in the life-extending effects of dietary restriction. RNAi of pha-4 decreases lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains. | 17476212, 19079239, 19239417 | GenDR |
Data Type 2
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
Organism | Ageing Factor | Lifespan Observation | Reference | |||||||||||||||||||||||||||||||||||||||
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# | Observation Stable ID | Species | Strain | Gene | Compound | Other Ageing Factor | Significant Lifespan Effect | Change | Observed Lifespan | Reference Lifespan | Lifespan Unit | Measure | Temperature | Temperature Unit | Sex/Mating Type | Description | Author | Year | PubMed | DOI | Source | |||||||||||||||||||||
1 | OB_002780 | Caenorhabditis elegans | N2 |
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increased | 37.14% | 19.2 | 14 | days | mean | 20 | ℃ | hermaphrodite | Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR. | Ching et al. | 2010 | 20456299 | doi:10.1111/j.1474-9726.2010.00580.x | Lifespan Observations Database (ID: 4072) | |||||||||||||||||||||||
2 | OB_003923 | Caenorhabditis elegans |
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increased | 25.00% | RNAi decreased lifespan approximately 25% Other phenotypes: PHA-4 may play a role in the life-extending effects of dietary restriction. RNA interference decreased lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains. Overexpression slightly increased longevity. | Panowski et al. | 2007 | 17476212 | doi:10.1038/nature05837 | Lifespan Observations Database (ID: 637) | |||||||||||||||||||||||||||||||
3 | OB_003924 | Caenorhabditis elegans |
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increased | 23.00% | Overexpression increased lifespan by 23% Other phenotypes: PHA-4 may play a role in the life-extending effects of dietary restriction. RNA interference decreased lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains. Overexpression slightly increased longevity. | Panowski et al. | 2007 | 17476212 | doi:10.1038/nature05837 | Lifespan Observations Database (ID: 638) | |||||||||||||||||||||||||||||||
4 | OB_002778 | Caenorhabditis elegans | N2 |
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increased | 18.57% | 16.6 | 14 | days | mean | 20 | ℃ | hermaphrodite | Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR. | Ching et al. | 2010 | 20456299 | doi:10.1111/j.1474-9726.2010.00580.x | Lifespan Observations Database (ID: 4070) | |||||||||||||||||||||||
5 | OB_002775 | Caenorhabditis elegans | N2 |
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increased | 7.27% | 17.7 | 16.5 | days | mean | 20 | ℃ | hermaphrodite | Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR. | Ching et al. | 2010 | 20456299 | doi:10.1111/j.1474-9726.2010.00580.x | Lifespan Observations Database (ID: 4068) | |||||||||||||||||||||||
6 | OB_003399 | Caenorhabditis elegans | N2 |
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none | 0.00% | 15 | 15 | days | mean | 20 | ℃ | There was no significant difference in lifespan with RNAi knockdown of pha-4. | Lucanic et al. | 2011 | 21562563 | doi:10.1038/nature10007 | Lifespan Observations Database (ID: 4821) | ||||||||||||||||||||||||
7 | OB_004263 | Caenorhabditis elegans | N2 |
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decreased | -5.03% | 15.1 | 15.9 | days | mean | 25 | ℃ | hermaphrodite | RNAi knock-down of pha-4 significantly decreased the life span of N2 and raga-1(ok386) animals, but did not completely prevent life span extension from deletion of raga-1. | Schreiber et al. | 2010 | 20523893 | doi:10.1371/journal.pgen.1000972 | Lifespan Observations Database (ID: 958) | |||||||||||||||||||||||
8 | OB_002776 | Caenorhabditis elegans | N2 |
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decreased | -10.91% | 14.7 | 16.5 | days | mean | 20 | ℃ | hermaphrodite | Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR. | Ching et al. | 2010 | 20456299 | doi:10.1111/j.1474-9726.2010.00580.x | Lifespan Observations Database (ID: 4069) | |||||||||||||||||||||||
9 | OB_005716 | Caenorhabditis elegans |
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decreased | -13.00% | Average | RNAi of pha-4 in adult animals led to a slightly shortened mean lifespan (13%). | Sheaffer et al. | 2008 | 18804378 | doi:10.1016/j.cub.2008.07.097 | GenAge | ||||||||||||||||||||||||||||||
10 | OB_004262 | Caenorhabditis elegans | N2 |
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decreased | -13.84% | 13.7 | 15.9 | days | mean | 25 | ℃ | hermaphrodite | RNAi knock-down of pha-4 significantly decreased the life span of N2 and raga-1(ok386) animals, but did not prevent life span extension from deletion of raga-1. | Schreiber et al. | 2010 | 20523893 | doi:10.1371/journal.pgen.1000972 | Lifespan Observations Database (ID: 957) | |||||||||||||||||||||||
11 | OB_003398 | Caenorhabditis elegans | N2 |
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decreased | -31.82% | 15 | 22 | days | mean | 20 | ℃ | Lifespan extension resulting from faah-1 overexpression requires the Foxa transcription factor PHA-4. | Lucanic et al. | 2011 | 21562563 | doi:10.1038/nature10007 | Lifespan Observations Database (ID: 4820) | ||||||||||||||||||||||||
12 | OB_005715 | Caenorhabditis elegans |
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decreased | unknown | PHA-4 may play a role in the life-extending effects of dietary restriction. RNA interference decreased lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains. Overexpression slightly increased longevity. | Panowski et al. | 2007 | 17476212 | doi:10.1038/nature05837 | GenAge |
Homology Analysis
no homology analysis observation available
Sequences
PHA4_CAEEL | Q17381 | Defective pharyngeal development protein 4 (Caenorhabditis elegans) from UniProtKB/Swiss-Prot Length: 506 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 PHA4_CAEEL 1 MTSPSSDEDIIDIRVIKEEPESEPDSEAEPATTTNSTDSEDSVEQENKKLLETEKNRKREQKHKMLPNGTTSGTSDTGNQVPATSSAASSVDYTAMNAQDYLPTYSNTTLNYQPYQYQTAANGLLNYNNYSQYATANQLGSNYISPANFMQGGGISPLGFTTGTTGATTAAASVATSSASAVIGRSNGRSSSTVAASPADRSYSGVSGGQGQELTIQEFETVTEKIRRHGTYGQSKPPYSYISLITMAIQKSNSRQLTLSEIYNWIMDLFPYYQNNQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSFWTLHEHCGNMFENGCYLRRQKRFKVKEREPSRKKRNANSQQLHQQQHIPKMEIKEEDPTSITTTSSLGAYSLIPQISTKKEIKEELKAVQDATAAAANLGLIDPSGTPSAVNHSQPTSVISSVGTLGTTQAQMTLNGQYASPYLYSSDFATILPQSQNFLNNTLYNTTSSYPGIDYTNGVYQNTLYSSTNPNSAANL 506
References
MeSH Terms: Only a subset of the Medical Subject Headings terms is shown: the Major Topics MeSH terms. They describe one of the main topics discussed in the article denoted by an asterisk on the MeSH term or MeSH/Subheading combination on the PubMed page. MeSH terms that belong to the Ageing MeSH are highlighted in green.
- Panowski SH, Wolff S, Aguilaniu H, Durieux J, Dillin A:
PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans.
Nature. 2007; 447: 550-5.
PubMed (ID: 17476212), doi:10.1038/nature05837
MeSH Terms: Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans/physiology; Caloric Restriction; Diet; Longevity/physiology; Trans-Activators/metabolism - Sheaffer KL, Updike DL, Mango SE:
The Target of Rapamycin pathway antagonizes pha-4/FoxA to control development and aging.
Curr Biol. 2008; 18: 1355-64.
PubMed (ID: 18804378), PubMed Central (ID: PMC2615410), doi:10.1016/j.cub.2008.07.097
MeSH Terms: Caenorhabditis elegans Proteins/antagonists & inhibitors; Caenorhabditis elegans Proteins/physiology; Caenorhabditis elegans/physiology; Phosphotransferases (Alcohol Group Acceptor)/physiology; Trans-Activators/antagonists & inhibitors - Honjoh S, Yamamoto T, Uno M, Nishida E:
Signalling through RHEB-1 mediates intermittent fasting-induced longevity in C. elegans.
Nature. 2009; 457: 726-30.
PubMed (ID: 19079239), doi:10.1038/nature07583
MeSH Terms: Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans/metabolism; Fasting/physiology; Longevity/physiology; Monomeric GTP-Binding Proteins/metabolism; Signal Transduction - Greer EL, Brunet A:
Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans.
Aging Cell. 2009; 8: 113-27.
PubMed (ID: 19239417), PubMed Central (ID: PMC2680339), doi:10.1111/j.1474-9726.2009.00459.x
MeSH Terms: Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caloric Restriction/methods; Food Deprivation/physiology; Food, Formulated; Longevity/physiology; Signal Transduction/genetics - Ching TT, Paal AB, Mehta A, Zhong L, Hsu AL:
drr-2 encodes an eIF4H that acts downstream of TOR in diet-restriction-induced longevity of C. elegans.
Aging Cell. 2010; 9: 545-57.
PubMed (ID: 20456299), PubMed Central (ID: PMC2910166), doi:10.1111/j.1474-9726.2010.00580.x
MeSH Terms: Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans/physiology; Caloric Restriction; Diet; Eukaryotic Initiation Factors/metabolism; Longevity/physiology; Phosphotransferases (Alcohol Group Acceptor)/metabolism - Schreiber MA, Pierce-Shimomura JT, Chan S, Parry D, McIntire SL:
Manipulation of behavioral decline in Caenorhabditis elegans with the Rag GTPase raga-1.
PLoS Genet. 2010; 6: e1000972.
PubMed (ID: 20523893), PubMed Central (ID: PMC2877737), doi:10.1371/journal.pgen.1000972
MeSH Terms: Behavior, Animal; Caenorhabditis elegans/physiology; GTP Phosphohydrolases/metabolism - Lucanic M, Held JM, Vantipalli MC, Klang IM, Graham JB, Gibson BW, Lithgow GJ, Gill MS:
N-acylethanolamine signalling mediates the effect of diet on lifespan in Caenorhabditis elegans.
Nature. 2011; 473: 226-9.
PubMed (ID: 21562563), PubMed Central (ID: PMC3093655), doi:10.1038/nature10007
MeSH Terms: Caenorhabditis elegans/physiology; Diet; Ethanolamines/metabolism; Longevity/physiology; Signal Transduction
Sources
Ageing-related Data Sources
- AgeFactDB Curator
- GenAge
- GenDR
- Lifespan Observations Database
Additional Data Sources
- AgeFactDB Pipeline
- GPSDB
- NCBI Taxonomy
- PubMed
- UniProtKB/Swiss-Prot