Gene: Indy - Drosophila melanogaster
Last modified: Dec 09, 2014. Release 1 • Page created: May 19, 2024
Summary
Gene Symbol |
: |
Indy |
NCBI Gene ID |
: |
40049 |
Species |
: |
Drosophila melanogaster |
Gene Synonyms |
: |
CG3979 | CG3979-PA | CG3979-PB | CG3979-PC | CG3979-PD | Dmel_CG3979 | DmelCG3979 | drIndy | I am not dead yet | I'm not dead yet | INDY | Indy | indy | Indy-PA | Indy-PB | Indy-PC | Indy-PD
Source: GPSDB |
Gene Homologs |
: |
AgaP_AGAP007054 (Anopheles gambiae) | AgaP_AGAP007055 (Anopheles gambiae) | Indy (Drosophila melanogaster) | Os08g0503700 (Oryza sativa) | Os09g0484900 (Oryza sativa) | SLC13A2 (Bos taurus) | SLC13A2 (Canis lupus familiaris) | slc13a2 (Danio rerio) | SLC13A2 (Gallus gallus) | SLC13A2 (Homo sapiens) | SLC13A2 (Macaca mulatta) | Slc13a2 (Mus musculus) | SLC13A2 (Pan troglodytes) | Slc13a2 (Rattus norvegicus) | TDT (Arabidopsis thaliana) |
Ageing Relevance Analysis |
: |
Observation Type |
Count |
Ageing Relevance |
Source |
Ageing Phenotype Observation—Data Type 1 |
1 |
yes (Exp. Analysis) |
GenDR |
Ageing Phenotype Observation—Data Type 2 Lifespan Effect |
|
|
|
increased |
1 |
yes (Exp. Analysis) |
Lifespan Observations Database |
Homology Analysis |
1 |
- (basis for a Comp. Analysis) |
AgeFactDB Homology Analysis |
Total |
3 |
|
|
|
Ageing Factor Stable ID |
: |
AF_007642 |
Download |
: |
XML |
Protein Information
Observations
Ageing Phenotype
Data Type 1
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
# |
Observation Stable ID |
Species |
Gene |
Other Ageing Factor |
Description |
PubMed |
Source |
1 |
OB_005425 |
Drosophila melanogaster |
Gene Symbol |
NCBI Gene ID |
Indy |
40049 |
|
|
Flies heterozygotic for a disruption have extended mean (87-92%) lifespan and maximum lifespan (45%). Homozygotes for the disruption show only a 10-20% increase in mean lifespan. |
11118146 |
GenAge |
2 |
OB_007997 |
Drosophila melanogaster |
Gene Symbol |
NCBI Gene ID |
Indy |
40049 |
|
Other Ageing Factor Name |
Dietary restriction |
|
Flies heterozygotic for a disruption in Indy gene have extended mean (87-92%) and maximum (45%) lifespan. Homozygotes for the disruption show only a 10 - 20% increase in mean lifespan [ 11118146]. Heterozygous insertion of a p-element in the non-coding region of Indy locus leads to a reduction in Indy mRNA expression and causes a significant median lifespan extension in male and female by about 29% and 34%, respectively. At normal or high calorie conditions Indy heterozygote mutants have a significant lifespan extension, but under low calorie conditions, Indy heterozygous mutants have minimal median lifespan extension. Reduction of calorie content from high to normal calorie condition results in 19% decline in Indy mRNA and from normal to low calorie condition results in additional 9% decrease in Indy mRNA. Reduction of calorie content from high to normal calorie conditions in heterozygous Indy mutants leads to 20% reduction in Indy mRNA expression without any additional decrease upon further reduction to low calorie food. Maximum lifespan extension is associated with Indy mRNA levels between 25 - 75% of normal. Long-lived heterozygous Indy mutants on high-calorie food and normal wild-type on low-calorie food have several phenotypes in common: 50 - 60 % reduced mRNA expression levels of Ilp2, Ilp3 and Ilp 5; similar high percentage of anti-FOXO-positive nuclei in fat body cells; higher sensitivity to starvation; do not gain weight; similar decrease in triglycerides and fat storage; normal food intake [ 19470468]. Mutations in Indy dramatically extend lifespan without a loss in fertility, physical activity, flight velocity or metabolic rate [11118146; 12626742]. Indy encodes a high-affinity dicarboxylate/citrate plasma membrane transporter found most abundantly in adult fat body, oenocytes and midgut cells, the primary sites of intermediary metabolism [ 12391301]. Indy mutation alters metabolism in a manner similiar to DR and mutants have several phenotypes with long-lived DR files in common, including decreases insulin-like signaling, lipid storage, weight gain, and resistance to starvation, and an increase in spontaneous physical activity [ 19470468]. Of the Indy206 and Indy302 mutation only one of the two has lower mRNA levels and both do not extend lifespan of female flies in any genetic background. In original genetic background only Indy mutation associated with altered RNA expression extends the lifespan of males. This effect is abolished by back-crossing into standard out-bred genetic backgrounds and is associated with an unidentified locus on the X chromosome. Original Indy line with long-lived males is infected by the cytoplasmic Wolbachia. Longevity of Indy males disappear after tetracycline clearance of this endosymbiont [ 17571923]. |
19470468 |
GenDR |
Data Type 2
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
Homology Analysis
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
Legend:- #EGHG:
- Number of Experimentally Confirmed Ageing-related Genes In Homology Group
# |
Observation Stable ID |
Homology Group Genes |
#EGHG |
Description |
Source |
Homology Source |
1 |
OB_008209 |
|
1 |
The HomoloGene homology group 2965 contains 1 gene with experimental evidence for ageing relevance (Indy - Drosophila melanogaster) and 14 other genes. |
AgeFactDB Homology Analysis |
HomoloGene homology group 2965 |
Sequences
References
MeSH Terms:
Only a subset of the Medical Subject Headings terms is shown: the Major Topics MeSH terms.
They describe one of the main topics discussed in the article denoted by an asterisk on the MeSH term or MeSH/Subheading combination on the PubMed page.
MeSH terms that belong to the Ageing MeSH are highlighted in green.
- Rogina B, Reenan RA, Nilsen SP, Helfand SL:
Extended life-span conferred by cotransporter gene mutations in Drosophila.
Science. 2000; 290: 2137-40.
PubMed (ID: 11118146)
MeSH Terms: Aging/genetics; Carrier Proteins/genetics; Dicarboxylic Acid Transporters; Drosophila Proteins; Drosophila melanogaster/genetics; Genes, Insect; Longevity/genetics; Organic Anion Transporters, Sodium-Dependent; Symporters
- Wang B, Goode J, Best J, Meltzer J, Schilman PE, Chen J, Garza D, Thomas JB, Montminy M:
The insulin-regulated CREB coactivator TORC promotes stress resistance in Drosophila.
Cell Metab. 2008; 7: 434-44.
PubMed (ID: 18460334), PubMed Central (ID: PMC3704161), doi:10.1016/j.cmet.2008.02.010
MeSH Terms: Cyclic AMP Response Element-Binding Protein/metabolism; Drosophila Proteins/metabolism; Hypoglycemic Agents/pharmacology; Insulin/pharmacology; Oxidative Stress; Transcription Factors/metabolism
- Wang PY, Neretti N, Whitaker R, Hosier S, Chang C, Lu D, Rogina B, Helfand SL:
Long-lived Indy and calorie restriction interact to extend life span.
Proc Natl Acad Sci USA. 2009; 106: 9262-7.
PubMed (ID: 19470468), PubMed Central (ID: PMC2685744), doi:10.1073/pnas.0904115106
MeSH Terms: Aging/genetics; Caloric Restriction; Dicarboxylic Acid Transporters/genetics; Dicarboxylic Acid Transporters/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/physiology; Symporters/genetics; Symporters/metabolism
Sources
Ageing-related Data Sources
Additional Data Sources