Gene: FEN1 - Homo sapiens
Last modified: Dec 09, 2014. Release 1 • Page created: May 19, 2024
Summary
Gene Symbol | : | FEN1 | ||||||||||||||||
NCBI Gene ID | : | 2237 | ||||||||||||||||
Species | : | Homo sapiens | ||||||||||||||||
Gene Synonyms | : | DNase IV | FEN-1 | FEN1 | flap endonuclease 1 | FLAP STRUCTURE-SPECIFIC ENDONUCLEASE 1 | flap structure-specific endonuclease 1 | MATURATION FACTOR 1 | maturation factor 1 | maturation factor-1 | MF1 | RAD2 Source: GPSDB |
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Gene Homologs | : | AgaP_AGAP011448 (Anopheles gambiae) | crn-1 (Caenorhabditis elegans) | FEN1 (Arabidopsis thaliana) | FEN1 (Bos taurus) | FEN1 (Canis lupus familiaris) | Fen1 (Drosophila melanogaster) | FEN1 (Gallus gallus) | FEN1 (Homo sapiens) | FEN1 (Macaca mulatta) | Fen1 (Mus musculus) | FEN1 (Pan troglodytes) | Fen1 (Rattus norvegicus) | KLLA0F02992g (Kluyveromyces lactis) | MGG_03419 (Magnaporthe oryzae) | NCU10776 (Neurospora crassa) | Os05g0540100 (Oryza sativa) | rad2 (Schizosaccharomyces pombe) | RAD27 (Saccharomyces cerevisiae) | ||||||||||||||||
Ageing Relevance Analysis | : |
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Ageing Factor Stable ID | : | AF_008582 | ||||||||||||||||
Download | : | XML |
Protein Information
Protein Name | Species | UniProt Accession Number | UniProt Entry Name | Protein Function | Sequence |
---|---|---|---|---|---|
Flap endonuclease 1 | Homo sapiens | P39748 (UniProtKB/Swiss-Prot) | FEN1_HUMAN (UniProtKB/Swiss-Prot) | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. | View |
FEN1 protein | Homo sapiens | Q6FHX6 (UniProtKB/TrEMBL) | Q6FHX6_HUMAN (UniProtKB/TrEMBL) | View |
Observations
Ageing Phenotype
Data Type 1
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
# | Observation Stable ID | Species | Gene | Other Ageing Factor | Description | PubMed | Source | ||||
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1 | OB_009879 | Homo sapiens |
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Involved in DNA processing, FEN1 has been associated with cancer progression [0586]. A FEN1 role in ageing is unknown, but due to its interactions with WRN [0584] and PCNA [0588], FEN1 might, in theory, be involved in human ageing. | GenAge |
Data Type 2
no ageing phenotype observation—data type 2 available
Homology Analysis
Ageing Relevance:
- yes (Exp. Analysis)
- yes, but no ageing factor assigned (Exp. Analysis)
- no (Exp. Analysis)
- putative (Comp. Analysis)
Legend:
- #EGHG:
- Number of Experimentally Confirmed Ageing-related Genes In Homology Group
# | Observation Stable ID | Homology Group Genes | #EGHG | Description | Source | Homology Source |
---|---|---|---|---|---|---|
1 | OB_008389 | 2 | The HomoloGene homology group 3034 contains 2 genes with experimental evidence for ageing relevance (FEN1 - Homo sapiens | RAD27 - Saccharomyces cerevisiae) and 16 other genes. | AgeFactDB Homology Analysis | HomoloGene homology group 3034 |
Sequences
FEN1_HUMAN | P39748 | Flap endonuclease 1 (Homo sapiens) from UniProtKB/Swiss-Prot Length: 380 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 FEN1_HUMAN 1 MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK 380
Q6FHX6_HUMAN | Q6FHX6 | FEN1 protein (Homo sapiens) from UniProtKB/TrEMBL Length: 380 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 Q6FHX6_HUMAN 1 MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK 380
References
MeSH Terms: Only a subset of the Medical Subject Headings terms is shown: the Major Topics MeSH terms. They describe one of the main topics discussed in the article denoted by an asterisk on the MeSH term or MeSH/Subheading combination on the PubMed page. MeSH terms that belong to the Ageing MeSH are highlighted in green.
- Harrington JJ, Lieber MR:
The characterization of a mammalian DNA structure-specific endonuclease.
EMBO J. 1994; 13: 1235-46.
PubMed (ID: 8131753), PubMed Central (ID: PMC394933)
MeSH Terms: DNA/chemistry; Endodeoxyribonucleases/isolation & purification; Endodeoxyribonucleases/metabolism; Nucleic Acid Conformation; Oligodeoxyribonucleotides/metabolism - Liu Y, Bambara RA:
Analysis of human flap endonuclease 1 mutants reveals a mechanism to prevent triplet repeat expansion.
J Biol Chem. 2003; 278: 13728-39.
PubMed (ID: 12554738), doi:10.1074/jbc.M212061200
MeSH Terms: Endodeoxyribonucleases/genetics; Mutation - Hosfield DJ, Mol CD, Shen B, Tainer JA:
Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.
Cell. 1998; 95: 135-46.
PubMed (ID: 9778254)
MeSH Terms: DNA Repair; DNA Replication; Endodeoxyribonucleases/chemistry; Proliferating Cell Nuclear Antigen/metabolism - Otto CJ, Almqvist E, Hayden MR, Andrew SE:
The "flap" endonuclease gene FEN1 is excluded as a candidate gene implicated in the CAG repeat expansion underlying Huntington disease.
Clin Genet. 2001; 59: 122-7.
PubMed (ID: 11260214)
MeSH Terms: Exodeoxyribonucleases/genetics; Flap Endonucleases; Huntington Disease/genetics; Trinucleotide Repeat Expansion/genetics - Hasan S, Stucki M, Hassa PO, Imhof R, Gehrig P, Hunziker P, Hübscher U, Hottiger MO:
Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300.
Mol Cell. 2001; 7: 1221-31.
PubMed (ID: 11430825)
MeSH Terms: Endodeoxyribonucleases/genetics; Endodeoxyribonucleases/metabolism; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcriptional Activation/physiology - Brosh RM, von Kobbe C, Sommers JA, Karmakar P, Opresko PL, Piotrowski J, Dianova I, Dianov GL, Bohr VA:
Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity.
EMBO J. 2001; 20: 5791-801.
PubMed (ID: 11598021), PubMed Central (ID: PMC125684), doi:10.1093/emboj/20.20.5791
MeSH Terms: DNA Helicases/physiology; Endodeoxyribonucleases/metabolism; Werner Syndrome/genetics - Hoopes LL, Budd M, Choe W, Weitao T, Campbell JL:
Mutations in DNA replication genes reduce yeast life span.
Mol Cell Biol. 2002; 22: 4136-46.
PubMed (ID: 12024027), PubMed Central (ID: PMC133874)
MeSH Terms: Adenosine Triphosphatases/genetics; DNA Helicases/genetics; DNA Replication/genetics; DNA-Binding Proteins; Saccharomyces cerevisiae Proteins; Silent Information Regulator Proteins, Saccharomyces cerevisiae; Yeasts/physiology - Kucherlapati M, Yang K, Kuraguchi M, Zhao J, Lia M, Heyer J, Kane MF, Fan K, Russell R, Brown AM, Kneitz B, Edelmann W, Kolodner RD, Lipkin M, Kucherlapati R:
Haploinsufficiency of Flap endonuclease (Fen1) leads to rapid tumor progression.
Proc Natl Acad Sci USA. 2002; 99: 9924-9.
PubMed (ID: 12119409), PubMed Central (ID: PMC126601), doi:10.1073/pnas.152321699
MeSH Terms: Adenocarcinoma/genetics; Endodeoxyribonucleases/deficiency; Endodeoxyribonucleases/genetics; Intestinal Neoplasms/genetics; Mutation - Huggins CF, Chafin DR, Aoyagi S, Henricksen LA, Bambara RA, Hayes JJ:
Flap endonuclease 1 efficiently cleaves base excision repair and DNA replication intermediates assembled into nucleosomes.
Mol Cell. 2002; 10: 1201-11.
PubMed (ID: 12453426)
MeSH Terms: Base Pair Mismatch; DNA Repair; DNA/chemistry; Endodeoxyribonucleases/physiology; Nucleosomes/metabolism - Caldecott KW:
DNA single-strand break repair and spinocerebellar ataxia.
Cell. 2003; 112: 7-10.
PubMed (ID: 12526788)
MeSH Terms: DNA Damage/genetics; DNA Repair/genetics; DNA-Binding Proteins/genetics; Nuclear Proteins/genetics; Polynucleotide 5'-Hydroxyl-Kinase/genetics; Spinocerebellar Ataxias/genetics
Sources
Ageing-related Data Sources
- AgeFactDB Homology Analysis
- GenAge
Additional Data Sources
- AgeFactDB Pipeline
- GPSDB
- HomoloGene
- NCBI Taxonomy
- PubMed
- UniProtKB/Swiss-Prot
- UniProtKB/TrEMBL