Other Ageing Factor: Dietary restriction - Aedes aegypti, Caenorhabditis elegans, Caenorhabditis remanei, Ceratitis capitata, Danio rerio, Drosophila melanogaster, Mus musculus, Nothobranchius furzeri, Podospora anserina, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe

Gene Symbol	NCBI Gene ID
aak-2	181727

Other Ageing Factor Name
Dietary restriction

AAK-2 could be a sensor that couples energy levels and insulin-like signals to lifespan. aak-2(ok524) knockout mutants have a 12% and 18% shorter mean and maximum lifespan, respectively as well as faster age-dependent accumulation of a lipofuscin-like fluorescent pigment in the intestine [

15574588]. sDR increases AMP:ATP ratio. aak-2 mutation suppresses lifespan extension and delay of the decline in locomotor activity resulting from sDR. A constitutive active mutation of aak-2 is sufficient to cause increase stress resistance as well as to significantly extend lifespan. Both increased stress resistance and extended lifespan is reverted in daf-16 knockdown by RNAi. sod-3 mRNA is increased by constitutive active form of aak-2 and decreased by aak-2 mutation. The increase in sod-3 mRNA is dependent on expression of DAF-16. Worm and human AMPK phosphorylate DAF-16 (greatly enhanced by presence of AMP) at least in six residues (T166, S202, S314, S321, T463 and S466) [

17900900]. aak-2 mutation cancels out the lifespan extension effect of sDR and PD, regardless of the concentration of bacteria or peptones. bDR significantly extends lifespan of aak-2 mutants, but to lesser extent than that of wild-type. eat-2 mutation extends the lifespan of aak-2 mutants to the same extent than that of wild-type. Resveratrol does not increase lifespan of aak-2 mutants [

19239417]. daf-2(m577);aak-2(ok524) double mutant has a lifespan that is indistinguishable from those of aak-2(ok524) single mutant. Transgenic animals with a higher aak-2 gene dose live on average 13% longer with a maximum lifespan extension on up to 25% [

Gene Symbol	NCBI Gene ID
aakg-2	181736

Other Ageing Factor Name
Dietary restriction

aakg-2 overexpression extends mean, median, and maximum lifespan by 47, 45, and 35%. Overexpression of aakg-2 toegther with D. rerio ucp2 was non-additive with sDR [

22737090].

OB_007916

Gene Symbol	NCBI Gene ID
AAT1	853755

Other Ageing Factor Name
Dietary restriction

Overexpression of AAT1 extends replicative lifespan by 25% and does not synergize with 0.5% glucose restriction [

Gene Symbol	NCBI Gene ID
ADE4	855346

Other Ageing Factor Name
Dietary restriction

ade4 mutation extends chronological lifespan, but not replicative lifespan, and is non-additive with 0.5% glucose or amino-acid DR on chronological lifespan extension. ADE4 deletion in atg16 mutants results only in a partial extension of the chronological lifespan by 0.5% glucose DR [

Gene Symbol	NCBI Gene ID
age-1	174762

Other Ageing Factor Name
Dietary restriction

Recessive knockout mutants of age-1 have a 40-65% increase in mean lifespan and a 65-110% increase in maximum lifespan [8608934; 8700226]. age-1(mg44) zygotic null mutants have a mean (99%) and maximum (117%) lifespan extension [

18828672]. Even in axenic culture lifespan of age-1 is extended up to 100%. age-1 mutation significantly extends lifespan under AL, but only slightly under sDR [

16720740]. RNAi against age-1 extends lifespan by 30% [8700226; 8608934]. age-1 RNAi increases mean and maximum lifespan by 36-46% and 48-50% [

12447374]. RNAi against age-1 increases mean lifespan by 83% [

18828672]. age-1 mutants are dauer constitutive [

8056303] and display lower brood size as well as increased embryonic lethality [

9504918]. Additionally, age-1 mutants have elevated levels of superoxidase dismutase and catalase activities [

8389142]. age-1 RNAi and mutation extend lifespan by 30% and 100%, respectively [

8608934, 8700226].

16720740

OB_007919

Gene Symbol	NCBI Gene ID
AIM4	852493

Other Ageing Factor Name
Dietary restriction

AIM4 (alias SOY1) deletion increases chronological and replication lifespan, which is non-additive with DR. On AL mean and maximum replicative lifespan are extended by 63 and 69%, respectively. DR appears to decrease aim4-induced replication lifespan extension, indicating a negative interaction. aim4 mutation does not change DR-induced chronological lifespan extension [

OB_007920

Gene Symbol	NCBI Gene ID
Akh	38495

Other Ageing Factor Name
Dietary restriction

Knockdown of the adipokinetic hormone (Akh) by RNAi (with an RU486-inducible and ubiquitously expressing Actin 5C-GS Gal4 strain) does not by itself affect lifespan, but significantly inhibits DR-dependent yhe increase in lifespan across a range of yeast concentrations in both females and males. While control females and males exhibit a 113%/22% increase in lifespan under DR, upon Akh inhibition there was a significant reduction in lifespan extension with DR (52%/5%). Global Akh knockdown reduces starvation resistance by 24% upon DR, but no significant change upon AL. Also Akh RNAi repressed the DR-dependent increase in cold-stress resistance. Fat body and neuronal-specific inhibition of Akh by using RU486-inducible S(1)106-GS-Gal4 and Elav-GS-Gal4 enhancer traps, respectively, does not reduce lifespan extension upon DR. But, muscle-specific inhibition of Akh using RU486-inducible muscle enhancer trap (Mhc-GS-Gal4) reduces the DR-dependent increase in lifespan. While control exhibit a 47.2% lifespan extension, animals with muscle-specific Akh inhibition fails to result in any increase upon DR (i.e. completely blocked the DR lifespan extension). Muscle-specific Akh inhibition diminishes the increase in triglyceride synthesis and breakdown present normally under DR. A significant reduction in lifespan extension also occurs with a noninducible muscle driver (Mhc-Gal4). Controls on DR exhibit significant higher levels of spontaneous activity compared to Akh RNAi-inhibited animals at all ages. Akh inhibition reduces the protective effect of DR on age-related decline in muscle function/activity [Katewa et al. 2012]. Fat-body specific Akh RNAi results in increased spontaneous activity and a small but significant increase in lifespan upon AL [Katewa et al. 2012]. Overexpression of Akh in a ubiquitousness manner enhances fat metabolism (significant increase in triglyceride synthesis and breakdown under AL), spontaneous activity (148% on AL and 154% on DR), and lifespan on Al (33%). However, despite and increase in movement under DR, lifespan is not increased under a restricted diet [Katewa et al. 2012].

OB_007921

Gene Symbol	NCBI Gene ID
aqp-1	174190

Other Ageing Factor Name
Dietary restriction

aqp-1 expression changes in response to glucose or glycerol. Similar to daf-16 and hsf-1 mutants, aqp-1 mutants were short-lived, and their short lifespan was not further decreased by glucose. Overexpression of aqp-1::GFP rescues short lifespan of aqp-1 deletion mutants and partially prevented glucose from shortening lifespan. Glucose or glycerol feeding downregulates aqp-1 in wild-type. In daf-16 and/or hsf-1 mutants aqp-1 is repressed and glucose feeding does not significantly affect its expression. aqp-1 mutation does not further decrease the short lifespan of daf-16 and/or hsf-1 mutants. aqp-1 transgene is expressed in pharynx and intestine (which behaves as entire endoderm of animal, including adipose tissues). Dietary glucose does not cause significant differences in levels of glucose or glycerol in wild-type vs. aqp-1 mutants [

Gene Symbol	NCBI Gene ID
atf1	2540329

Other Ageing Factor Name
Dietary restriction

Activation of transcription factor Atf1 by Sty1 is required for chronological lifespan extension and enhanced heat stress resistance by DR. Deleting atf1 cancels out DR-mediated chronological lifespan extension and enhanced heat stress resistance. Overexpressing atf1 is not sufficient to promote chronological lifespan extension in cells lacking sty1 [

Gene Symbol	NCBI Gene ID
atg-7	178005

Other Ageing Factor Name
Dietary restriction

RNA interference against atg-7 shortens mean lifespan by 23% and maximum lifespan by 30% in a eat-2 mutant background but not in wild-type animals. Thus, atg7 RNAi does not affect lifespan of wild-type, but totally cancels out the lifespan extension effect of eat-2 mutation [

Gene Symbol	NCBI Gene ID
ATG10	850684

Other Ageing Factor Name
Dietary restriction

ATG10 deletion cancels out replicative lifespan extension by DR [

OB_007925

Gene Symbol	NCBI Gene ID
ATG11	856162

Other Ageing Factor Name
Dietary restriction

ATG11 deletion extends replicative lifespan under AL and abrogates DR-lifespan extension [

OB_007926

Gene Symbol	NCBI Gene ID
ATG15	850432

Other Ageing Factor Name
Dietary restriction

Deletion of ATG15 does not affect the lifespan significantly on AL, while DR shortens replicative lifespan of ATG15 deletion mutant by 28% [

Gene Symbol	NCBI Gene ID
ATG16	855194

Other Ageing Factor Name
Dietary restriction

Under AL atg16 mutation shortens chronological, but not replicative lifespan. 0.5% glucose DR extends chronological lifespan of atg16 mutants, but amino-acid DR does not extend the short chronological lifespan of atg16 mutants (similar to several other autophagy mutants). ADE4 deletion in atg16 mutants results only in a partial extension of chronological lifespan by 0.5% glucose DR. The long chronological lifespan of tor1 mutants requires ATG16 [

Gene Symbol	NCBI Gene ID
ATG17	851142

Other Ageing Factor Name
Dietary restriction

ATG17 deletion decreases replicative lifespan under AL and blocks DR-lifespan extension. ATG17 mutant's replicative lifespan decreases by 70% on DR [

OB_007929

Gene Symbol	NCBI Gene ID
ATG2	855479

Other Ageing Factor Name
Dietary restriction

ATG2 deletion prevents chronological lifespan extension induced by amino-acid DR [

Gene Symbol	NCBI Gene ID
bec-1	177345

Other Ageing Factor Name
Dietary restriction

bec-1 is required for normal dauer morphogenesis and lifespan extension. Knockdown of bec-1 via RNA interference results in a shortened mean and maximum lifespan by 14 and 5% [

12958363]. bec-1 RNAi does not significantly change the lifespan of wild-type, but completely suppresses the longevity phenotype of eat-2 mutation [17912023; 18282106] and prevents lifespan extension by daf-2(e1370) mutation [

12958363]. bec-1 RNAi causes the formation of abnormal dauers in a daf-2(e1379) background [

Gene Symbol	NCBI Gene ID
Bmcp	39322

Other Ageing Factor Name
Dietary restriction

bmcp (ucp5) knockout extends lifespan under DR more than that of WT, but no longer on starvation or high-calorie diet. Ectopic neuronal expression of bmcp transgene rescues starvation sensitive phenotype of bmcp knockout mutants (1638786).

1638786

OB_007932

Gene Symbol	NCBI Gene ID
BMH1	856924

Other Ageing Factor Name
Dietary restriction

Deleting BMH1 extends chronological lifespan by 25% and is associated with activated stress response, decreased ROS levels and increased heat-shock-element-driven transcription activity. BMH1 deletion was non-additive with the genetic DR mimetic cdc25 and tor1. Water starvation (a form of extreme DR) extends chronological lifespan of BMH1 mutant even more as it does in wild-type. BMH1 genetically interacts with DR as well as TOR- and PKA-signaling pathways to regulate lifespan. Phosphorylation of Ser238 on Bmh1 increases during chronological aging, which is delayed by DR or reduced TOR activity [

19805817].

19805817

OB_007933

Gene Symbol	NCBI Gene ID
BMH2	851676

Other Ageing Factor Name
Dietary restriction

Overexpressing 14-3-3 protein, Bmh2 significantly extends CLS (19805817).

19805817

OB_007934

Gene Symbol	NCBI Gene ID
cbp-1	176380

Other Ageing Factor Name
Dietary restriction

bDR and daf-2 mutation induce cbp-1 expression. There is no decrease in cbp-1 expression in whole C. elegans during aging. Overexpression of cbp-1 does not significantly affect lifespan. daf-16 RNAi and cbp-1 RNAi reduce average lifespan under AL to about the same extent. Inhibiting cbp-1 via RNAi by 50%, specifically in adult phase and completely blocks lifespan extension of DD, bDR as well as eat-2, glp-1 and clk-1 mutation, but only partially that of daf-2 mutation and not at all that of cold. cbp-1 RNAi completely blocks the lifespan increase by daf-2 mutation under bDR. cbp-1 RNAi blocks the delay of other age-related pathologies by bDR. cbp-1 RNAi prevents protective effects of bDR and accelerates ABeta42-related pathology. bDR significantly delays onset of paralysis even in presence of cbp-1 RNAi. cbp-1 RNAi specifically in adults completely blocks lifespan extension by three distinct protocols of DR (mutation of eat-2), partly by daf-2 mutation but not of cold and blocks the delay of other age-related pathologies by bDR. cbp-1 RNAi has no effect on lifespan in daf-16 hypomorphic mutants. Combining cbp-1 and daf-16 RNAi in wild-type produces similar lifespan as either alone. Resistance to oxidative stress is strikingly reduced by cbp-1 RNAi and cbp-1 RNAi attenuates the protection against oxidative stress by bDR. cbp-1 RNAi accelerates accumulation of autofluorescence, but has no effect on activity, egg laying, or pharyngeal pumping. cbp-1 RNAi does not block induction of daf-16 or hsf-1 by bDR, but does block the induction of DAF-16 target gene, sod-3, and HSF-1 target gene, sip-1 by bDR. cbp-1 RNAi blocks induction of sod-3 expression by daf-2 RNAi. cbp-1 RNAi does not block the increased Nile Red staining produced by daf-2 mutation, but enhanced Nile Red staining. cbp-1 RNAi blocks the effect of bDR on metabolic gene expression from glycolysis towards beta-oxidation. Drugs that enhance histone acetylation increase lifespan and reduce ABeta42-related pathologies, but these protective effects are completely blocked by cbp-1 RNAi. cbp-1 RNAi decreases H4 Lys 5 acetylation and blocks the extension of lifespan as well as delays the onset of paralysis by ABeta1-42 transgene under AL and bDR by sodium butyrate (NaB) and trichostatin (TSA). cbp-1 RNAi does produce dye-filling defects in all C. elegans amphid neurons (ASI, ADL, ASK, AWB, ASH, and ASJ) [

Gene Symbol	NCBI Gene ID
Cbs	33081

Other Ageing Factor Name
Dietary restriction

Ubiquitous or neuron-specific transgenic overexpression of Cbs enhances longevity in fully-fed animals. Cbs RNAi partially abrogates increased lifespan by DR, but has no effect on fully fed animals. Cbs upregulation is required for increased lifespan under low-nutrient conditions. Response of male flies to DR is muted in comparison with females. Adult-specific ubiquitous expression of Cbs is sufficient to increase female mean and maximum lifespan by 12 - 43% and 10%, respectively. Males, whose lifespan is relatively less affected by DR, exhibite a smaller, but still significant increase in lifespan by 7% upon Cbs overexpression. Neuronal overexpression also increases lifespan, albeit modestly (approximately 12% mean and 15% maximum lifespan extension), whereas overexpression in the fat body and in the gut has no effect [

21930912].

OB_007936

Gene Symbol	NCBI Gene ID
CDC25	851019

Other Ageing Factor Name
Dietary restriction

The CDC25-10 allele extends mean and maximum replicative lifespan by 34% and 18%, respectively, at 30 degree Celsius. cdc25-10 mutants have an extended replicative lifespan under AL. Growth on 0.5% glucose restriction does not further extend replicative lifespan of cdc25-10 mutants. CDC25 null mutant is not viable. CDC25 appears to act in the same genetic pathway as SIR2 and NPT1 and is suggested to be genetic model of DR [

Gene Symbol	NCBI Gene ID
CoVb	33918

Other Ageing Factor Name
Dietary restriction

CG11015 is translational upregulated upon DR. Under rich nutritional conditions lifespan of CG11015 RNAi treated animals is indistinguishable from that of controls, while upon DR, lifespan extension is diminished in males and females [

Gene Symbol	NCBI Gene ID
CG5389	39761

Other Ageing Factor Name
Dietary restriction

RNAi of complex V subunit CG5389 results in increased mean longevity under standard laboratory food conditions (3% yeast) in males [

19747824]. Under rich media conditions CG5389 knockdown throughout development and adulthood increases mean lifespan by 26% and abolished the lifespan extension by DR (started in the adulthood) in males. Suppression of CG5389 only during the adulthood either via RNAi by tub-GS or via oligomycin (a specific inhibitor of complex V) feeding prevents an increase in longevity under DR (started in the adulthood) in males [

19968629].

OB_007939

Gene Symbol	NCBI Gene ID
CG7834	34276

Other Ageing Factor Name
Dietary restriction

Muscle specific RNAi knockdown of CG7834 which reduces its mRNA levels by 25-35%, significantly reduces the DR-dependent lifespan extension. CG7834 RNAi animals exhibit only a 14% increase compared to the 55% lifespan-increase in controls upon DR [Katewa et al. 2012].

OB_007940

Gene Symbol	NCBI Gene ID
CG7834	43515

Other Ageing Factor Name
Dietary restriction

OB_007941

Gene Symbol	NCBI Gene ID
cha-1	13192683

Other Ageing Factor Name
Dietary restriction

cha-1 encodes a choline acetyltransferase which is expressed in motor [

18041778] neurons and downregulated in space. Mutation or RNA interference of cha-1 extends lifespan on NGM agar covered with killed or live bacteria as well as in liquid culture medium [

22768380]. cha-1(TY1652) mutation extends mean, 75%ile, and maximum lifespan by 23, 29, and 38%. The cha-1(PR1152) allele extends mean, 75%ile, and maximum lifespan by 22-49, 18-25, and 11-21%. Lifespan extension by cha-1 mutation is not abolished by daf-16 RNAi inactivation. eat-2 RNAi shortens the lifespan of cha-1 mutants. RNAi inactivation of cha-1 reduces Q35 aggregation [

22768380]. cha-1 participates in determining pharyngeal pumping rate to affect food intake [

6698395].

OB_007942

Gene Symbol	NCBI Gene ID
chico	64880

Other Ageing Factor Name
Dietary restriction

Mutation in chico extends mean, median, and maximum lifespan by 56%, 48%, and 42% in homozygotes and 44%, 36%, and 35% in heterozygotes. chico mutation produces dwarf, long-lived females at normal nutrition. Male heterozygous live 13% longer than wild-type, but male homozygous have a shortened lifespan [

11292874]. Wild-type and chico mutant females have similar peak lifespan under DR, but the food concentration at which these are achieved is shifted to higher amounts. chico mutation induces a state equivalent to submaximal, DR-induced slowing of aging [

11951037]. chico heterzoygous females have a reduced fecundity and homozygous recessive mutants are sterile. chico heterozygous mutants are resistant to starvation but not oxidative stress or temperature stress [

11292874].

11292874, 11951037

OB_007943

Gene Symbol	NCBI Gene ID
ckr-1	188774

Other Ageing Factor Name
Dietary restriction

ckr-1 RNAi significantly reduces lifespan of eat-2 but not that of age-1 nor clk-1 mutants [

Gene Symbol	NCBI Gene ID
ckr-2	175341

Other Ageing Factor Name
Dietary restriction

ckr-2 RNAi significantly reduces the lifespan of eat-2 but not that of age-1 nor clk-1 mutants [

Gene Symbol	NCBI Gene ID
clk-1	175729

Other Ageing Factor Name
Dietary restriction

Mutations in clk-1 slow down development and extend lifespan by 30%. Mutation of both clk-1 and daf-2 results in nearly 5-fold (500%) increase in lifespan [

8638122]. Food restriction by eat-2 mutation does not further extend the long lifespan of clk-1 mutant [

9789046]. DR and clk-1 mutations may extend lifespan by a similar process. DR by intermittent fasting (IF) significantly extends lifespan of clk-1 mutants, but to a lesser extent than that of wild-type [

19079239]. clk-1 mutants do not respond to sDR-induced lifespan extension [

19239417]. Overexpression of clk-1 shortens lifespan and is associated with increased mitochondrial activity [

10202142]. clk-1 encodes a enzyme participating in coenzyme Q synthesis [9020081; 11136229]. clk-1 mutants have a decreased pharyngeal pumping and may provoke volunteering DR [

9789046]. Mutations in clk-1 are highly pleiotropic resulting in an average lengthing of embryonic development, post-embryonic development, and adult rhythmic behaviours such as defecation, swimming and pharyngeal pumping [

7768437]. clk-1 mutants require coeznyme Q [

11136229]. clk-1 protein binds the mitochondrial O(L) region and may regulate replication of mitochondrial DNA [

11959146].

9789046, 19079239, 19239417

OB_007946

Gene Symbol	NCBI Gene ID
cup-4	176104

Other Ageing Factor Name
Dietary restriction

cup-4 RNAi or overexpession reduces oxidative stress resistance and shortens lifespan of wild-type under AL. cup-4 RNAi significantly reduces the extended lifespan of eat-2 mutants, but failed to block lifespan extension of age-1 or clk-1 mutants. Lifespan of cup-4 mutants increases only moderately by sDR [

Gene Symbol	NCBI Gene ID
cup-5	176074

Other Ageing Factor Name
Dietary restriction

cup-5 RNAi significantly decreases lifespan of eat-2 specifically, whereas lifespan of age-1 or clk-1 mutants are not affected [

Gene Symbol	NCBI Gene ID
CYR1	853452

Other Ageing Factor Name
Dietary restriction

The CDC35-1 allele of the adenylate cyclase CYR1 confers a 75% extension of replicative lifespan at 25 degree Celsius [

11000115]. Transposon-mutagenized CYR1 increases resistance to oxidants and extends chronological lifespan by up to 90%. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 are required for this lifespan extension [

11292860]. CYR1 mutation is assumed to act as genetic DR mimetic [

Gene Symbol	NCBI Gene ID
CYT1	854231

Other Ageing Factor Name
Dietary restriction

Deletion of CYT1 decreases RLS and cancels RLS extension of HAP4 overexpression (12124627). Initially, it was shown that deletion of CYT1 also prevents life span extension by glucose DR (14724176). But later it was shown that either 0.5 or 0.05 % glucose DR increases life span of cytDelta cells (16311627).

12124627, 14724176, 16311627

OB_007950

Gene Symbol	NCBI Gene ID
daf-16	172981

Other Ageing Factor Name
Dietary restriction

Mutations in daf-16 suppresses life-extension caused by mutations in daf-2 [

8247153]. daf-16 is required for lifespan extension by mutation of daf-2 or age-1 [

8247153]. RNAi against daf-16 decreases lifespan of wild-type, daf-2 or glp-1 mutants [22509016; 16530050]. Loss of function alleles of daf-16 shorten lifespan, but some alleles have lifespan equal to wild-type [

8247153]. daf-16 mutation significantly reduces lifespan under AL (-20%), but does not prevent lifespan extension by sDR. In another experiment daf-16 mutation totally suppresses lifespan extension by sDR [

16720740]. sDR does not stimulate DAF-16 translocation to the nucleus, but daf-16 mutation cancels out the ability of sDR to extend lifespan and to delay the decline in locomotor activity [

17900900]. DR by bacterial dilution extends lifespan of daf-16 mutants [

17538612]. daf-16 mutation decreases lifespan under AL, but fails to prevent bDR to further extend lifespan [

18331616]. IF-induced lifespan-extension by either 24h/48h/72h per 4 days is significantly diminished in null mutants of daf-16. All these regimens extend lifespan of daf-16 to a lesser extent than that of wild-type. daf-16 partially mediates IF-induced longevity [

19079239]. Glucose or glycerol does not shorten lifespan of daf-16 mutants [

19883616]. daf-16 mutation cancels out the lifespan extension effect of sDR and PD, regardless of the concentration of bacteria or peptones. bDR significantly extends lifespan of daf-16 mutants, but to a lesser extent than that of wild-type. eat-2 mutation extends the lifespan of daf-16 mutants to the same extent than that of wild-type. Resveratrol extends lifespan of daf-16 mutants [

19239417]. daf-16 RNAi completely blocks the lifespan extension by daf-2 mutation, but only partially by bDR. daf-16 RNAi attenuates protection against oxidative stress by bDR. daf-16 expression is induced by bDR [

19924292]. Knockdown of daf-16 decreases mean and maximum lifespan by 50% and 54%, respectively [

22509016]. DAF-16 reduces expression of rsks-1 and daf-15 [15253933; 22560223]. daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan [

18828672]. Overexpression of wild-type DAF-16 modestly increases lifespan by 20% [

11747825], while overexpression of constitutive nuclear forms of DAF-16 increases lifespan only slightly [

11381260]. daf-16(mu86) mutation decreases mean (44%) and maximum (18%) lifespan [

15905404]. daf-16(mgDf47) decreases mean (18-37%) and maximum (29%) lifespan [

18828672]. daf-16 mutants are dauer defective [

7219552] and completely suppress all the phenotypes of daf-2 and age-1 mutations, including lifespan extension, dauer arrest, reduced fertility, and viability defects [8247153; 7789761; 9504918; 7789761]. Mutations in daf-16 also suppress lifespan extension of animals that have a germ line ablation [

10360574]. Sex-specific lifespan potential requires daf-16 [

10747056]. daf-16 mutation suppresses enhanced UV resistance as well as increase longevity of daf-2, daf-23, spe-26, and clk-1 mutants. Mutation in daf-16 does not alter the reduced fertility in spe-26. daf-16 mutants are more fertile than wild-type [

8807294].

16720740, 17538612, 17900900, 18331616, 19079239, 19239417, 19883616, 19924292

OB_007951

Gene Symbol	NCBI Gene ID
daf-2	175410

Other Ageing Factor Name
Dietary restriction

daf-2 mutants live more than twice as long as controls. daf-2(sa189) mutation extends mean and maximum lifespan by 133 and 129%, respectively, when shifted to 20 degree Celsius. The daf-2(e1370) mutation extends mean and maximum lifespan by 32 and 119%, respectively, when shifted to 25 degree Celsius and by 110 and 145%, respectively, at 20 degree Celsius. daf-2(sa189) mutation extends mean lifespan by 67% as well as maximum lifespan [

8247153]. This lifespan extension requires the activity of daf-16 [

8247153]. The lifespan extension of daf-2(e1370) mutants is cancelled out by daf-16(m26) mutation. daf-2 mutants still exhibit a long lifespan after ablation of the gonad and germ cells. [

8247153]. daf-2(e1370) increases mean (95-118%) and maximum (165%) lifespan [

18828672]. RNAi against daf-2 extends mean and maximum lifespan by 47 and 65% [

12471266]. daf-2 mutation extends lifespan of wild-type and eat-2 mutants [

9789046]. Long lifespan of daf-2 insulin receptor mutation is further extended by sDR. However, daf-2 mutation is not a null mutation, therefore it is still possible that part of sDR-induced increase in lifespan might depend on insulin receptor pathway [

17900900]. DR by bacterial dilution extends lifespan of daf-2 mutants [

17538612]. IF does not markedly extend lifespan of daf-2 mutants [

19079239]. 2% glucose reduce fractions of animals that become dauers at 22.5 degree Celsius in daf-2 mutants. Glucose almost completely suppresses lifespan extension of daf-2 ligand binding domain and tyrosine kinase mutants back to wild-type levels [

19883616]. daf-2 mutation increases average lifespan by 157%. Under AL daf-2 mutation increases lifespan by 30%. bDR increases lifespan by 65%. daf-2 mutation further increases lifespan under bDR by 40%. Resistance to oxidative stress is reduced daf-2 mutation [

19924292]. daf-2 RNAi increases mean lifesapn by 89% [

18828672]. daf-2(m577) mutation increases mean and maximum lifespan by 33 and 29%, respectively, while daf-2(e1370) mutation increases mean and maximum lifespan by 101 and 181%, respectively [

16782295]. DR from eat-2(ad465) mutation has an addative effect on lifespan of daf-2(e1370) adults, but not on lifespan of daf-2(e1368) adults [

18043747]. Mutation in daf-2 in combination with mutation of daf-12 results in nearly 300% increase in lifespan [

7789761]. daf-2 mutants are dauer constitutive [

7219552] and exhibit reduced brood size [9504918; 9725835]. daf-2 mutants synergize with germ line ablation for lifespan extension [

10360574] and also exhibit synergy with clk-1 mutation for lifespan prolongation [

8638122]. All the phenotypes of daf-2 mutants are suppressed by mutation of daf-16 [8247153; 8601482; 7789761; 9725835; 9504918]. Mutation of daf-2 increases expression of sod-3 [

10428762].

9789046, 17538612, 17900900, 19079239, 19883616, 19924292

OB_007952

Gene Symbol	NCBI Gene ID
DAP2	856423

Other Ageing Factor Name
Dietary restriction

DAP2 deletion decreases mean and maximum replicative lifespan under AL by 19 and 36%, respectively, and cancels out the lifespan extending effect of moderate DR [Wuttke et al., unpublished].

OB_007953

Gene Symbol	NCBI Gene ID
drr-1	174994

Other Ageing Factor Name
Dietary restriction

RNAi of drr-1 starting at L1 extends mean, 25%ile and median lifespan by 3, 8 and 8% [

15998808]. drr-1 RNAi significantly extends mean lifespan of wild-type by 37 - 44%, of daf-16 mutants by 18%, and of daf-2 mutants by 14%, but does not extend significantly the lifespan of eat-2 mutants. drr-1 RNAi does not affect pumping, but similar to DR reduces and delays reproduction as well as causes a slender appearance. drr-1 mRNA is 2-fold downregulated in response to DR [

Gene Symbol	NCBI Gene ID
drr-2	259546

Other Ageing Factor Name
Dietary restriction

RNA interference of drr-2 extends lifespan [

15998808]. drr-2 RNAi extends lifespan of wild-type by 10-16%, but fails to significantly extend lifespan of daf-2 mutants or eat-2 mutants. drr-2 RNAi keeps a normal, well-fed appearance and normal reproduction. drr-2 mRNA expression is 2-fold reduced in eat-2 mutants [

16103914]. drr-2 RNAi significantly reduces paralysis in Q35YFP transgenic animals [

18331616]. drr-2 overexpression suppresses lifespan extension by eat-2 mutation and solid plate-based DR [

Gene Symbol	NCBI Gene ID
dve-1	180398

Other Ageing Factor Name
Dietary restriction

dve-1 RNAi attenuates lifespan extension by bDR, but only partially that of daf-2 mutation. dve-1 RNAi attenuates protection against oxidative stress by bDR. dve-1 expression is not induced by bDR [

19924292].

19924292

OB_007956

Gene Symbol	NCBI Gene ID
eat-2	175072

Other Ageing Factor Name
Dietary restriction

eat-2 mutants life significant longer by up to 57 % (9789046). eat-2 RNAi significnatly reduces paralysis in Q35YFP or A?42 transgenic animals (18331616). sDR further increase the long lifespan of eat-2 mutants (Greer and Brunet, 2009)

9789046, 18331616, 19239417

OB_007957

Gene Symbol	NCBI Gene ID
egl-9	179461

Other Ageing Factor Name
Dietary restriction

egl-9 deletion does not affect lifespan under AL. Lifespan extension under modified sDR regimen is diminished by egl-9 mutation. egl-9 mutation significantly suppresses the lifespan extension by a strong loss-of-function allele of eat-2. Lifespan extension by deletion mutants of rsks-1 is fully suppressed by egl-9 mutation [

Gene Symbol	NCBI Gene ID
Eip55E	37143

Other Ageing Factor Name
Dietary restriction

Propargylglycine (PPG) inhibits gamma-cystathioinase, the second enzyme of the trans-sulfuration pathway (TSP). PPG is a specific suicidal inhibitor of gamma-cystathionase Eip55E. Gluthatione (GSH) levels are decreased by PPG administration in flies subjected to DR, whereas there is no effect on fully fed animals. PPG robustly suppresses DR lifespan extension, while longevity of fully fed flies is not affected in different strains. Thus, indicating that the effect of PPG is specific to DR. PPG abrogates changes in lifespan that are normally observed when flies are maintained in different dietary concentrations and compositions [

21930912].

OB_007959

Gene Symbol	NCBI Gene ID
elt-3	181503

Other Ageing Factor Name
Dietary restriction

Expression of elt-3 decreases with development and aging. elt-3 RNAi extends maximum lifespan and totally cancels out the daf-2 or DR-induced (eat-2) lifespan extension [

18662544].

OB_007960

Gene Symbol	NCBI Gene ID
ERG3	850745

Other Ageing Factor Name
Dietary restriction

Deletion of ERG3 decreases replicative lifespan under AL, cancels out replicative lifespan extension of 0.5% glucose DR and results under DR also into a shorter replicative lifespan than under AL [

18690010

OB_007961

Gene Symbol	NCBI Gene ID
ERG5	855029

Other Ageing Factor Name
Dietary restriction

Deletion of ERG5 decreases replicative lifespan by 35% in the a strain [

18340043], but increases mean chronological lifespan by 26 - 116% (26, 40, 43, 62, 116) in diploid cells [

21447998]. Deletion of ERG5 cancels out the replicative lifespan extension of 0.5% glucose restriction [

Gene Symbol	NCBI Gene ID
ERG6	855003

Other Ageing Factor Name
Dietary restriction

Deletion of ERG6 cancels out replicative lifespan extension of 0.5% glucose DR and results under DR also into a shorter replicative lifespan than under AL [

18690010].

18690010

OB_007963

Gene Symbol	NCBI Gene ID
F57A8.4	186430

Other Ageing Factor Name
Dietary restriction

F57A8.4 encodes a rhodopsin-like G-protein coupled receptor that is known to sense light [

11493519] and is downregulated in space. Mutation or RNA interference of F57A8.4 extends lifespan on NGM agar covered with killed or live bacteria as well as in liquid culture medium. F57A8.4 RNAi extends the mean, 75%ile and maximum lifespan by 34, 39, and 61%. F57A8.4(tm4341) mutation extends the mean, 75%ile, and maximum lifespan by 18-38, 21-25, and 42-68%. Lifespan extension by gar-3 mutation is not abolished by RNAi inactivation of either daf-16 nor skn-1. eat-2 RNAi shortens the lifespan of F57A8.4 mutants [

22768380]. Mutation and RNAi of F57A8.4 suppresses pheromone-induced dauer formation [

22768380].

OB_007964

Gene Symbol	NCBI Gene ID
faah-1	177613

Other Ageing Factor Name
Dietary restriction

faah-1 overexpression reduces eicosapentaenoyl ethanolamide (EPEA), palmitoleyol ethanolamide, linoleyol ethanolamide, as well as arachidonoyl ethanolamide (AEA) levels, delays development, increases thermal stress resistance, and was associated with mean and maximum adult lifespan extension by 19 and 35%, respectively, in presence of abundant food but not under (two different protocols of) DR. Overexpression in pharynx was largely sufficient for this lifespan extension [

21562563].

OB_007965

Gene Symbol	NCBI Gene ID
FET3	855080

Other Ageing Factor Name
Dietary restriction

FET3 mutation slightly shortens chronological lifespan under AL. Its chronological lifespan is not extended by 0.5% glucose or amino-acid DR [

20421943]. FET3 is one of several iron related genes that are up-regulated in response to increasing strength of glucose DR [

Gene Symbol	NCBI Gene ID
FKH1	854675

Other Ageing Factor Name
Dietary restriction

Deletion of FKH1 or FKH2 has no effect on neither replicative, nor chronological lifespan [

18225956]. Deletion of both FKH1 and FKH2 reduces mean chronological lifespan by 50% and abrogates lifespan extension and increased stress resistance conferred from water starvation (extreme DR). Modest increase in FKH1 or FKH2 expression results in a slight increased chronological and replicative lifespan as well as stress resistance [

22438832].

OB_007967

Gene Symbol	NCBI Gene ID
FKH2	855656

Other Ageing Factor Name
Dietary restriction

Deletion of FKH1 or FKH2 has no effect on neither replicative, nor chronological lifespan [

18225956]. Deletion of both FKH1 and FKH2 reduces mean chronological lifespan by 50% and abrogates lifespan extension and increased stress resistance conferred from water starvation (extreme DR). Modest increase in FKH1 or FKH2 expression results in slight increased chronological and replicative lifespan as well as stress resistance [

22438832].

OB_007968

Gene Symbol	NCBI Gene ID
foxo	41709

Other Ageing Factor Name
Dietary restriction

foxo overexpression extends lifespan. Activation of foxo in the adult pericerbral fat body is sufficient for lifespan extension. Limited activation of foxo reduces the expression of Drosophila insulin-like peptide dilp-2 synthesised in neurons and, represses endogenous insulin-dependent signaling in peripheral fat body [

15175753]. foxo is not required for DR, but its activity modulates the response. foxo null mutants are highly and significantly shorter-lived than wild-type on all food dilutions apart from 0.1 SY and under starvation. foxo null mutants are not more sensitive to starvation than wild-type. foxo overexpression in adult fat body under normal nutrition leads to extension of lifespan of females and causes a right shift of the response curve of lifespan to DR [

Gene Symbol	NCBI Gene ID
FRE6	850675

Other Ageing Factor Name
Dietary restriction

FRE6 deletion increases mean replicative lifespan by 14% and cancels out the lifespan extending effect of DR [Wuttke et al., unpublished].

OB_007970

Gene Symbol	NCBI Gene ID
Ghr	14600

Other Ageing Factor Name
Dietary restriction

Ghr knockouts (the so called Laron mice) are dwarfs with significantly extended lifespan by 40-50% [

12933651]. Ghr-/- mice are significantly longer lived as Ghr+/+ or Ghr+/- mice (by 40-50%) in both females and males [10875265; 19370397]. 30% DR fails to affect overall survival, average or median long-lifespan of Growth hormone receptor knockout (GHRKO) mice and increased maximal lifespan only in females. Insulin sensitivity in GHRKO mutants is greater than in wild-type and is not further increased by DR [

16682650]. Intermittent fasting also fails to extend the long lifespan of GHRKO mice [

19747233]. Lifespan of mice with a deletion in the Ghr gene live almost 5 years [

21123740]. In C57BL/6J this mutation increases life expectancy by 16 to 26% depending on gender [

12933651] and in mice of mixed genetic background the increases amounted to 36-55% [

9371826]. Serum levels of GH are elevated in mutant mice [

9371826] and mutants are smaller than wild-type. IGF-1 and IGFBP-3 levels are also reduced in Ghr mutant mice [

10875265]. The age-associated decline in memory retention is delayed in Ghr mutants [

11336996]. Overexpression of a growth hormone antagonist (a mutated growth hormone that competes with the endogenous one) has no effect on lifespan [

Gene Symbol	NCBI Gene ID
gig	40201

Other Ageing Factor Name
Dietary restriction

Overexpression of dTsc2 increased mean lifespan by 20% and at 25 degree and protects mutants from deleterious effects of rich food, as if mimicking the effect of DR (15186745).

15186745

OB_007972

Gene Symbol	NCBI Gene ID
GIS1	851670

Other Ageing Factor Name
Dietary restriction

Deletion of GIS1 increases replicative lifespan by 25% in the alpha strain [

19030232] and causes major although not complete reversion of chronological lifespan extension by 0.5% glucose restriction [

Gene Symbol	NCBI Gene ID
GIT3	2538770

Other Ageing Factor Name
Dietary restriction

GIT3 encodes a G protein-coupled receptor for glucose. Git3 deletion increases CLS in conditions where glucose consumption is not affected. Constitutive activation of Galpha subunit acting downstream of Git3 accelerates aging and inhibits effect of DR. Anti-aging effect of DR and git3 deletion mutation is accompanied by increased respiration and lower ROS production (19266076).

19266076

OB_007974

Gene Symbol	NCBI Gene ID
GPA2	856741

Other Ageing Factor Name
Dietary restriction

Deletion of GPA2 increases mean and maximum replicative lifespan by 40% and 26%, respectively [

11000115]. Deletion of GPA2 extends replicative lifespan by reducing cAMP-PKA activity and provides a genetic model for DR [

Gene Symbol	NCBI Gene ID
gpa2	2541720

Other Ageing Factor Name
Dietary restriction

gpa2 (alias git8) encodes the alpha subunit of a heterotrimeric G protein, which acts downstream of Git3. Git8 activity accelerates aging and inhibits the lifespan-extending effect of DR. Constitutive active mutation of gpa2 decreases chronological lifespan under AL (2% glucose) and almost completely cancels out the lifespan extending effect of DR (0.2% glucose) [

Gene Symbol	NCBI Gene ID
GPR1	851527

Other Ageing Factor Name
Dietary restriction

Deletion of GRP1 increases mean and maximum replicative lifespan by 41% and 26%, respectively. GRP1 deletion mutants have also longer chronological lifespan. Deletion of GPR1 extends replicative lifespan by reducing cAMP-PKA activity and provides a genetically model for DR [

Gene Symbol	NCBI Gene ID
GSH1	853344

Other Ageing Factor Name
Dietary restriction

Deletion of GSH1 confers deficiency in glutathione biosynthesis and further increases chronological lifespan under 0.5% glucose restriction, but does not extend chronological lifespan under 2% glucose [

18840459]. Therefore, GSH1 has a positive interaction with DR [

Gene Symbol	NCBI Gene ID
GTR1	854918

Other Ageing Factor Name
Dietary restriction

GTR1 deletion decreases mean and maximum replicative lifespan under AL by 36 and 51%, respectively, and cancels out the lifespan extending effect of DR [Wuttke et al., unpublished].

OB_007979

Gene Symbol	NCBI Gene ID
GUP1	852796

Other Ageing Factor Name
Dietary restriction

GUP1 deletion extends mean and maximum replicative lifespan by 32 and 30%, respectively, as well as chronological lifespan. DR-induced maximal replicative lifespan extension is not further increased by GUP1 deletion, while gup1 mutant displayed longer chronological lifespan under DR [

OB_007980

Gene Symbol	NCBI Gene ID
GUT2	854651

Other Ageing Factor Name
Dietary restriction

Overexpression of GUT2 extends replicative lifespan by 25% and does not synergize with 0.5% glucose restriction [

Gene Symbol	NCBI Gene ID
HAP4	853751

Other Ageing Factor Name
Dietary restriction

Overexpression of HAP4 from the ADH1 promoter extends lifespan of PSY316 strain approximately 40% under growth conditions favoring fermentation (2% glucose). Overexpression of HAP4 increases replicative lifespan, but is non-additive with 0.5% glucose restriction in lifespan extension. Lifespan extension by HAP4 overexpression requires SIR2 [

12124627]. HAP4 deletion suppresses replicative lifespan extension to 30% and 33% on 0.1% glucose and on elimination of non-essential amino acids, respectively [

20178842]. HAP4 overexpressing cells demonstrate a transcriptional response resembling cells undergoing diauxic shift, consume more oxygen, and exhibit increased Sir2-dependent transcriptional silencing at telomeres and rDNA [

Gene Symbol	NCBI Gene ID
HDA1	855710

Other Ageing Factor Name
Dietary restriction

Deletion of HDA1 has no effect on longevity under normal condition, but acts synergistically with CR to increase RLS (12213553).

OB_007983

Gene Symbol	NCBI Gene ID
HES1	854412

Other Ageing Factor Name
Dietary restriction

Deletion of HES1 (alias OSH5) extends replicative lifespan and is non-addative with moderate DR. Elevation of OSH5 levels by an ERG6 promoter reduces mean, median and maximum replicative lifespan by 25, 18 and 29% [Geber et al., unpublished].

OB_007984

Gene Symbol	NCBI Gene ID
HHF1	852294

Other Ageing Factor Name
Dietary restriction

HHF1 deletion extends mean and maximum replicative by 45 and 69%, respectively, as well as chronological lifespan. Chronological lifespan extension by HHF1 deletion and DR is non-synergistic. DR appears to extend replicative lifespan more when combined with hhf1 mutation, whereas DR does not change hhf1-induced replicative lifespan extension, suggesting a positive interaction [

OB_007985

Gene Symbol	NCBI Gene ID
hif-1	180359

Other Ageing Factor Name
Dietary restriction

hif-1 mutation does not suppress lifespan extension of bDR or eat-2 mutation [

19372390]. hif-1 deletion extends lifespan by 24% and inhibition of hif-1 by RNAi also extends adult lifespan. hif-1 mutation extends lifespan under AL, but does not further extend lifespan extension under modified sDR. Activation of hif-1 by egl-9 deletion diminishes lifespan extension by modified sDR. hif-1 acts independent of insulin-like signaling: Lifespan extension by hif-1 suppression does not require DAF-16, because inhibition of hif-1 by RNAi extends lifespan of wild-type and daf-16 null mutant to a similar level. hif-1 RNAi further extends the lifespan of daf-2 mutants. hif-1 is in the TOR pathway, downstream of S6K/rsks-1: Inhibition of hif-1 by RNAi does not further extend lifespan of daf-15 heterozygous mutants. Lifespan extension by deletion mutant of rsks-1 is fully suppressed by egl-9 mutation. hif-1 mutation does not further extend rsks-1 lifespan. pha-4 RNAi slightly reduces lifespan in wild-type and hif-1 mutants, but hif-1 mutation extends lifespan of animals treated with control or pha-4 RNAi to a similar level [

Gene Symbol	NCBI Gene ID
hsf-1	173078

Other Ageing Factor Name
Dietary restriction

RNA interference of hsf-1 suppresses normal dauer formation and life-extension due to insulin-like signaling [

14668486]. hsf-1 overexpression extends mean, median, and maximum lifespan by 37, 35, and 29%[

22737090]. hsf-1 RNAi abrogates lifespan extension by daf-2(e1370) mutation, but not eat-2(ad1116) or isp-1(qm150). HSF-1, like DAF-16, is required for daf-2 mutations to extend lifespan [

12750521]. A mutant allele of hsf-1 slightly decreases lifespan under AL, but cancels out the lifespan extension effect of bDR. hsf-1 RNAi also prevents lifespan extension by bDR. bDR significantly reduces paralysis of Q35YFP or ABeta42 transgenic animals and hsf-1 RNAi totally cancels this effect. DR confers a general protective effect against proteotoxicity and promotes longevity by a mechanism involving hsf-1 [

18331616]. Glucose or glycerol does not shorten the lifespan of hsf-1 mutants. Glucose treatment completely suppresses the long lifespan caused by hsf-1 overexpression [

19883616]. sDR extends the lifespan of hsf-1 mutant with a premature stop codon, that eliminates activation domain, and that of wild-type to a similar extent [

19239417]. hsf-1 RNAi attenuates lifespan extension by bDR, but only partially that of daf-2 mutation. hsf-1 RNAi attenuates protection against oxidative stress by bDR. hsf-1 expression is induced by bDR [

19924292]. RNAi of hsf-1 shortens median and maximum lifespan by approximately 35%. hsf-1 RNAi animals exhibit phenotypes associated with accelerated aging (as assyed by Nomarsky microscopy) [

12136014].

12750521, 18331616, 19239417, 19883616, 19924292

OB_007987

Gene Symbol	NCBI Gene ID
hsp-12.6	177778

Other Ageing Factor Name
Dietary restriction

hsp-12.6 loss-of-function mutation significantly extends lifespan under AL and significantly suppresses intermittent fasting (IF)-induced increase in lifespan, to a similar extend to that of daf-16 mutation. HSP-12.6 expression is induced by fasting in various tissues, including body wall muscle and neuronal systems. hsp-16.2 is one of downstream targets of DAF-16 in IF-induced longevity. The extent of IF-induced longevity in daf-16 hsp-12.6 double mutant is similar to that of single hsp-12.6 or daf-16 mutants. hsp-12.6 and daf-16 function in same signaling pathway. Low insulin/IGF-like signaling in daf-2 results in constitutive activation of DAF-16 and higher expression of hsp-12.6 [

Gene Symbol	NCBI Gene ID
HSP12	850532

Other Ageing Factor Name
Dietary restriction

HSP12 deletion slightly increases lifespan under AL and totally cancels out the lifespan extending effect of moderate DR. hsp12 deletion mutant has increased resistance to various stresses and significantly reduced recombination rate at rDNA loci [Alan Morgan, personal communication].

19732046

OB_007989

Gene Symbol	NCBI Gene ID
HST1	854086

Other Ageing Factor Name
Dietary restriction

Deletion of HST1 blocks the residual replicative lifespan extension by hxk2 mutant in a sir2;fob1;hst2 triple mutant background [

16051752]. However, DR can increases the replicative lifespan to a similar extent in sir2;fob1;hst1;hst2 quadruple mutant cells as in sir2;fob1 double mutant cells under 0.5, 0.05 and 0.005% glucose conditions and even by hxk2 deletion mutant [

16741098, 17129213].

16051752, 16741098, 17129213

OB_007990

Gene Symbol	NCBI Gene ID
HST2	856092

Other Ageing Factor Name
Dietary restriction

HST2 overexpression extends replicative lifespan. 0.5% glucose restriction does not increase lifespan of sir2;fob1;hst2 triple mutants [

16051752]. DR increases lifespan of all four sir2;fob1;hstX(X = sirtuin) triple mutants [

16741098, 17129213].

16051752, 16741098, 17129213

OB_007991

Gene Symbol	NCBI Gene ID
HXK2	852639

Other Ageing Factor Name
Dietary restriction

Deletion of HXK2 extends mean and maximum replicative lifespan by about 53% and 33%, respectively. Limiting glucose availability by mutating HXK2 significantly extends replicative lifespan and provides a genetically model of DR [

11000115]. HXK2 deletion increases oxygene consumption. Changes in gene expression HXK2 mutation are quite similar to those of dietary-restricted cells. In fact, HXK2 mutants have a transcriptional profile that significantly resembles DR cells and cell overexpressing HAP4 [

Gene Symbol	NCBI Gene ID
HXT17	855809

Other Ageing Factor Name
Dietary restriction

HXT17 mutation extends both replicative and chronological lifespan as well as cancels out DR-induced replicative and chronological lifespan extension. Mean and maximum replicative lifespan are extended by 27 and 49%, respectively [

OB_007993

Gene Symbol	NCBI Gene ID
IDH2	854303

Other Ageing Factor Name
Dietary restriction

Deleting IDH2 extends RLS (16293764).

OB_007994

Gene Symbol	NCBI Gene ID
Ilp2	39150

Other Ageing Factor Name
Dietary restriction

Ilp2 is transcriptional down-regulated in long-lived mutants. Ilp2 null mutants are significant longer-lived with a 8-13% longer median lifespan, but have a normal DR response. Ilp2 Ilp3 Ilp5 triple null mutants fail to have a normal response to DR. Their response is right shifted, with mutants shorter-lived compared to wild-type on low but longer-lived on high yeast concentrations [

Gene Symbol	NCBI Gene ID
Ilp3	39151

Other Ageing Factor Name
Dietary restriction

Ilp3 null mutants have a normal lifespan under AL and a normal DR response. Ilp2 Ilp3 Ilp5 triple null mutants fail to have a normal response to DR. Their response is right shifted, with mutants being shorter-lived compared to wild-type on low but longer-lived on high yeast concentrations [

Gene Symbol	NCBI Gene ID
Ilp5	2768992

Other Ageing Factor Name
Dietary restriction

Abundance of Ilp5 mRNA is reduced under DR. Ilp5 null mutants have a normal lifespan under AL and a normal DR response. Ilp2 Ilp3 Ilp5 triple null mutants fail to have a normal response to DR. Their response is right shifted, with mutants being shorter-lived compared to wild-type on low but longer-lived on high yeast concentrations [

Gene Symbol	NCBI Gene ID
Indy	40049

Other Ageing Factor Name
Dietary restriction

Flies heterozygotic for a disruption in Indy gene have extended mean (87-92%) and maximum (45%) lifespan. Homozygotes for the disruption show only a 10 - 20% increase in mean lifespan [

11118146]. Heterozygous insertion of a p-element in the non-coding region of Indy locus leads to a reduction in Indy mRNA expression and causes a significant median lifespan extension in male and female by about 29% and 34%, respectively. At normal or high calorie conditions Indy heterozygote mutants have a significant lifespan extension, but under low calorie conditions, Indy heterozygous mutants have minimal median lifespan extension. Reduction of calorie content from high to normal calorie condition results in 19% decline in Indy mRNA and from normal to low calorie condition results in additional 9% decrease in Indy mRNA. Reduction of calorie content from high to normal calorie conditions in heterozygous Indy mutants leads to 20% reduction in Indy mRNA expression without any additional decrease upon further reduction to low calorie food. Maximum lifespan extension is associated with Indy mRNA levels between 25 - 75% of normal. Long-lived heterozygous Indy mutants on high-calorie food and normal wild-type on low-calorie food have several phenotypes in common: 50 - 60 % reduced mRNA expression levels of Ilp2, Ilp3 and Ilp 5; similar high percentage of anti-FOXO-positive nuclei in fat body cells; higher sensitivity to starvation; do not gain weight; similar decrease in triglycerides and fat storage; normal food intake [

19470468]. Mutations in Indy dramatically extend lifespan without a loss in fertility, physical activity, flight velocity or metabolic rate [11118146; 12626742]. Indy encodes a high-affinity dicarboxylate/citrate plasma membrane transporter found most abundantly in adult fat body, oenocytes and midgut cells, the primary sites of intermediary metabolism [

12391301]. Indy mutation alters metabolism in a manner similiar to DR and mutants have several phenotypes with long-lived DR files in common, including decreases insulin-like signaling, lipid storage, weight gain, and resistance to starvation, and an increase in spontaneous physical activity [

19470468]. Of the Indy206 and Indy302 mutation only one of the two has lower mRNA levels and both do not extend lifespan of female flies in any genetic background. In original genetic background only Indy mutation associated with altered RNA expression extends the lifespan of males. This effect is abolished by back-crossing into standard out-bred genetic backgrounds and is associated with an unidentified locus on the X chromosome. Original Indy line with long-lived males is infected by the cytoplasmic Wolbachia. Longevity of Indy males disappear after tetracycline clearance of this endosymbiont [

Gene Symbol	NCBI Gene ID
ins-7	191688

Other Ageing Factor Name
Dietary restriction

RNA interference of ins-7 extends the mean lifespan by 55% at 20 degree Celsius in N2 rrf-3(pk1426) [

12845331]. ins-7 RNAi significantly extends lifespan under AL. Treating wild-type with 2% glucose produced pattern of gene expression that overlaps significantly with that produced by genetic inhibition of daf-16 activity in daf-2 mutants. This results in changes in expression of several insulin-like genes, including DAF-16 target gene ins-7. Addition of glucose triggers an increased ins-7:GFP expression. Glucose suppresses the extended lifespan by ins-7 RNAi [

19883616]. RNAi of ins-7 does not further extend the lifespan in daf-2 mutants [

12845331]. ins-7 is repressed in animals with reduced daf-2 activity and elevuated in animals with reduced daf-16 activity.

19883616

OB_007999

Gene Symbol	NCBI Gene ID
IPK1	851910

Other Ageing Factor Name
Dietary restriction

Deletion of IPK1 increases mean replicative lifespan by 41 - 40% in the alpha strain [16293764; 19030232]. IPK1 deletion extends mean and maximum replicative lifespan by 24 and 19%, respectively, and was non-synergistic with moderate DR [

OB_008000

Gene Symbol	NCBI Gene ID
ire-1	174305

Other Ageing Factor Name
Dietary restriction

ire-1 mutation reduces slightly the lifespan under AL, but reduces significantly the lifespan extension by DR. ire-1 mutant has a significantly reduced slope in mean lifespan versus food concentrations relative to wild-type. ire-1 mutation fully suppresses lifespan extension by hif-1 mutation under AL and DR conditions [

Gene Symbol	NCBI Gene ID
l(3)neo18	46260

Other Ageing Factor Name
Dietary restriction

l(3)neo18 (alias CG9762) is translational upregulated upon DR. Under rich nutritional conditions lifespan of l(3)neo18 RNAi knockout animals is indistinguishable from wild-type, while upon DR, lifespan extension is diminished in males and females [

Gene Symbol	NCBI Gene ID
LAT1	855653

Other Ageing Factor Name
Dietary restriction

LAT1 is suggested to play a role in lifespan extension of DR. Deleting LAT1 abolishes replicative lifespan extension induced by 0.5% and 0.05% glucose restriction. In contrast, overexpressing Lat1 extends replicative lifespan, and this lifespan extension was not further increased by 0.5% glucose restriction. Similar to DR, replicative lifespan extension by LAT1 overexpression largely requires mitochondrial respiration [

17200108]. Overexpressing LAT1 extends lifespan (20% mean lifespan increase) and this lifespan extension is not further increased by DR. Similar to DR, lifespan extension by Lat1 overexpression largely requires mitochondrial respiration indicating mitochondrial metabolism plays an important role in DR. Interestingly, LAT1 overexpression does not require the Sir2 family to extend lifespan. Lat1 is also a limiting longevity factor in non-dividing cells in that overexpressing LAT1 extends cell survival during prolonged culture at stationary phase.

17200108

OB_008003

Gene Symbol	NCBI Gene ID
LCB4	854342

Other Ageing Factor Name
Dietary restriction

Deletion of LCB4 increases replicative lifespan and cancels out replicative lifespan extension of 0.5% glucose DR [

18690010

OB_008004

Gene Symbol	NCBI Gene ID
let-363	172167

Other Ageing Factor Name
Dietary restriction

let-363 RNAi significantly extends the lifespan of wild-type, but does not further extend the long lifespan of eat-2 mutant [

17266679]. let-363 RNAi led to an average increase in lifespan of 8% in wild-type and 3% in eat-2 background. let-363 works synergistically with eat-2 mutation. Inhibition of let-363 leads to a phenotype similar to starved animals and modest increase in lifespan [

16720740]. DR fails to significantly extend lifespan of let-363 RNAi-treated animals [

19079239]. RNAi or mutation of let-363 results in a doubling of lifespan. RNAi of let-363 begun at hatching extends lifespan to the same extent as RNAi begun at the first day of adulthood. RNAi of let-363 fails to further extend the lifespan of daf-2(e1370) mutants [

14668850]. Lifespan extension of let-363 RNAi does not require daf-16, as mutations in daf-16 do not suppress the long-lived phenotype of let-363 RNAi animals [

14668850]. let-363 RNAi slightly extends lifespan of daf-16 mutation [

16720740]. RNAi of let-363 causes many phenotypes similiar to daf-2 mutation: lipid accumulation in intestinal cells, reduced fertility, and reduced viability due to embryonic/larval arrest [

14668850]. The let-363(h111) allele has no significant effect on lifespan [

15253933]. Disruption of let-363 by RNAi appears to incomplete as lifespan is not extended as much asin let-363 mutants [

14668850]. The let-363(h114) allele at 25.5 degree Celsius extends mean lifespan by 150% [

14668850]. The absence of LET-363 activity causes developmental arrest at the L3 stage [

12225660].

16720740, 17266679, 19079239

OB_008005

Gene Symbol	NCBI Gene ID
lgc-26	178554

Other Ageing Factor Name
Dietary restriction

lgc-26 RNAi significantly decreases lifespan of eat-2 mutants specifically, whereas lifespan of age-1 or clk-1 mutants are not affected [

Gene Symbol	NCBI Gene ID
MDH1	853777

Other Ageing Factor Name
Dietary restriction

Overexpression of MDH1 extends replicative lifespan by 25% and does not synergize with 0.5% glucose restriction [

Gene Symbol	NCBI Gene ID
mdt-15	175817

Other Ageing Factor Name
Dietary restriction

mdt-15(tm2182) mutation does not affect lifespan on ad libitum, but further increases the lifespan when combined with DR (starting at the 4th day of adulthood) even more as wild-type [

22132200].

OB_008008

Gene Symbol	NCBI Gene ID
MSN2	855053

Other Ageing Factor Name
Dietary restriction

Deletion of MSN2 and MSN4 extends replicative lifespan and is further extended by cyr1::mTn [

14741356]. Deletion of MSN2 and MSN4 does not significantly decrease chronological lifespan under AL, but attenuates chronological lifespan extension by water starvation and 0.5% glucose restriction [

18225956] as well as cancels out lifespan extension of cyr1::mTn [

14741356] and decreases chronological lifespan extension of ras2 deletion mutant [

12586694]. Simulatnous deletion of MSN2 and MSN4 has no effect on chronological lifespan, but prevents lifespan extension by RAS2 deletion [

12586694]. msn2 msn4 has no effect on replicative lifespan in PSY316, and does not prevent lifespan extension by DR [

11000115] or by high osmolarity [

12391171].

12586694, 14741356, 18225956

OB_008009

Gene Symbol	NCBI Gene ID
MSN4	853803

Other Ageing Factor Name
Dietary restriction

Deletion of MSN2 and MSN4 extends replicative lifespan and is further extended by cyr1::mTn [

18225956] as well as cancels out lifespan extension of cyr1::mTn [

14741356] and decreases chronological lifespan extension of ras2 deletion mutant [

12586694]. Simulatnous deletion of MSN2 and MSN4 has no effect on chronological lifespan, but prevents lifespan extension by RAS2 deletion [

12586694]. msn2 msn4 has no effect on replicative lifespan in PSY316, and does not prevent lifespan extension by DR [

11000115] or by high osmolarity [

12391171].

12586694, 14741356, 18225956

OB_008010

Gene Symbol	NCBI Gene ID
NDE1	855176

Other Ageing Factor Name
Dietary restriction

Overexpression of NDE1 and NDE2 increases intracellular NAD/NADH ratio by lowering NADH concentration and increases replicative lifespan by 20-25%. This lifespan extension is non-additive 0.5% glucose restriction [

14724176]. Deletion of NDE1 extends chronological lifespan [

Gene Symbol	NCBI Gene ID
NDE2	851474

Other Ageing Factor Name
Dietary restriction

Gene Symbol	NCBI Gene ID
NFU1	853826

Other Ageing Factor Name
Dietary restriction

NFU1 mutation slightly shortens the chronological lifespan under AL and the chronological lifespan of NFU1 mutants is not extended by 0.5% glucose DR [

Gene Symbol	NCBI Gene ID
nlp-7	184647

Other Ageing Factor Name
Dietary restriction

nlp-7 RNAi or overexpression reduces oxidative stress resistance and shortens lifespan of wild-type under AL. nlp-7 RNAi significantly reduces extended lifespan of eat-2 mutants, but failed to block lifespan extension of age-1 or clk-1 mutants. Lifespan of nlp-7 mutants increases only moderately by sDR [

19783783]. nlp-7 expression is induced under DR via the use of a chemically defined axenic medium [

17023606] and by sDR [

Gene Symbol	NCBI Gene ID
NNT1	850991

Other Ageing Factor Name
Dietary restriction

Deletion of NNT1 decreases mean and maximum lifespan by 9 and 19%. 0.5% glucose DR extends the mean and maximum lifespan of NNT1 deletion mutants by 35 and 40%. Overexpression of NNT1 by 5-fold extends mean and maximum replicative lifespan by 18 and 23%, which is approximately of the same magnitude as the lifespan extension obtained from DR. DR in NNT1 overexpression mutant fails to significantly affect the lifespan and only results in extended mean lifespan by 12% and reduced maximum lifespan by 11%. NNT1 overexpression increases rDNA silincing, whereas deletion decreases rDNA silencing. Overexpression of human nicotinamide N-methyltransferase also increases rDNA silencing [

Gene Symbol	NCBI Gene ID
NPT1	854384

Other Ageing Factor Name
Dietary restriction

Increased dosage of NPT1 increases SIR2-dependent silencing, stabilizes the rDNA locus and extends replicative lifespan by up to 60%. 0.5% glucose restriction does not significantly further increase replicative lifespan of NPT1 overexpression [

11884393]. NPT1 deletion decreases replicative lifespan by 50% [

17482543] as well as chronological lifespan [

17110466]. Deletion of NPT1 shortens the lifespan in W303R. Replicative lifespan extension of cdc25-10 mutation (assumed to act as a genetic DR-mimetic) is cancelled out by NPT1 deletion [

11000115]. NPT1 mutation results in loss of telomere and rDNA silencing [

10841563], an effect that is likely caused by a loss of SIR2 activty due to decreased NAD levels. Mutation of NPT1 is synthetical lethal with mutation of QPT1 [

Gene Symbol	NCBI Gene ID
NYV1	850782

Other Ageing Factor Name
Dietary restriction

Deletion of NYV1 cancels out replicative lifespan extension of 0.5% glucose restriction and results under DR also into a shorter replicative lifespan than under AL. Thus, NYV1 deletion blocks DR-lifespan prolongation [

Gene Symbol	NCBI Gene ID
OPT2	856324

Other Ageing Factor Name
Dietary restriction

OPT2 deletion increases mean and maximum replicative lifespan by 23 and 9%, respectively, and cancels out the lifespan-extending effect of DR [Wuttke et al., unpublished].

105

OB_008018

Gene Symbol	NCBI Gene ID
Orco	40650

Other Ageing Factor Name
Dietary restriction

Loss-of-function mutation in Or83b results in olfactory defects, altered adult metabolism, enhanced stress resistance, and life-extension. Fully fed female homozygous Or83b null mutants exhibit a 56% increase in median lifespan and a 30% increase in maximum lifespan. Males are also significantly longer-lived, though to a smaller degree and maximum lifespan is not extended. Heterozygous mutants of both sexes show an intermediate longevity. Lifespan of homozygous Or83b null mutants is further increased by DR, but the relative increase in median and mean longevity is significantly greater when mutants were maintained in well-fed conditions [

Gene Symbol	NCBI Gene ID
p53	2768677

Other Ageing Factor Name
Dietary restriction

Overexpression of WT Dmp53 during adult life has no significant effect on lifespan. Expression of dominant negative form of Dmp53 specifically in neurons extends lifespan (+58% in females and +32 in males) and increases resistance to oxidative stress, but not to starvation or heat stress, while not affecting egg production or physical activity. Dominant negative Dmp53 expression cancels lifespan extension effect of DR, low calorie-food (5 % SY). Muscle or fat body specific expression of a dominant negative form of Dmp53 as well as globally lack of Dmp53 decreases lifespan (16303568).

Gene Symbol	NCBI Gene ID
PCK1	853972

Other Ageing Factor Name
Dietary restriction

Loss of Pck1 activity blocks chronological lifespan extension caused by water starvation. Knockout of PCK1 dramatically reduces chronological lifespan in both water (extreme DR) and glucose-containing medium. pck-1-K514Q mutation which abrogates enzymatic activity of Pck1, just like SIR2 deletion, extends chronological lifespan in water. Deletion of SIR2 does not alter the lifespan of PCK1 deletion mutant, pck1-K514R, and pck1-K514Q mutants [

Gene Symbol	NCBI Gene ID
PGA3	854914

Other Ageing Factor Name
Dietary restriction

Low glucose condition induces expression and activity of plasma membrane NADH coenzyme Q reductase (PGA3). Overexpressing PGA3 extends both, RLS and CLS (19239415).

Gene Symbol	NCBI Gene ID
pha-4	180357

Other Ageing Factor Name
Dietary restriction

pha-4 is required for multiple forms of DR. RNAi of pha-4 completely cancels out the lifespan extension of eat-2 mutation. Mutants of pha-4 do not respond to bacterial DR. Therefore, loss of pha-4 completely blocks the response to varying food concentration. Moreover, pha-4 expression is increased in response to DR in wild-type. pha-4 overexpression increases longevity of wild-type only slightly, but significant that of daf-16 mutants. The response to DR involves the PHA-4-dependent expression of sod-1, sod-2 and sod-5. Reduction of pha-4 does not suppress the long lifespan of daf-2 mutants or animals with defective electron transport chain [

17476212]. IF significantly extends lifespan of pha-4 [

19079239]. sDR extends lifespan of mutants with a temperature sensitive allele of pha-4 or pha-4 RNAi knockdown, but not daf-16 RNAi [

19239417]. PHA-4 may play a role in the life-extending effects of dietary restriction. RNAi of pha-4 decreases lifespan of wild-type worms, but not of daf-2 mutants or of animals with defective electron transport chains.

17476212, 19079239, 19239417

110

OB_008023

Gene Symbol	NCBI Gene ID
phb-1	171768

Other Ageing Factor Name
Dietary restriction

phb-1 RNAi shortens lifespan of WT, aak-2, cep-1 (p53) and jnk-1 mutants, but increase lifespan of daf-2, daf-7, daf-4, daf-11, gas-1, mev-1, isp-1, clk-1, nhr-49, fat-7, gld-1 mutants. daf-16 mutation fully suppresses exceptionally long-lifespan of via RNAi prohibitin-depleted, daf-2 mutants (19812672).

Gene Symbol	NCBI Gene ID
phb-2	174034

Other Ageing Factor Name
Dietary restriction

phb-2 RNAi shortens lifespan of WT, aak-2, cep-1 (p53) and jnk-1 mutants, but increase lifespan of daf-2, daf-7, daf-4, daf-11, gas-1, mev-1, isp-1, clk-1, nhr-49, fat-7, gld-1 mutants. daf-16 mutation fully suppresses exceptionally long-lifespan of via RNAi prohibitin-depleted, daf-2 mutants (19812672).

Gene Symbol	NCBI Gene ID
pka1	2539781

Other Ageing Factor Name
Dietary restriction

pka1 knockouts exhibits a three-fold increase in chronological lifespan with up to 187% longer maximum lifespan [

16822282]. Deleting ser/thr cAMP-activated protein kinase pka1 extends chronological lifespan under normal condition, but there is no additive effect with DR [

Gene Symbol	NCBI Gene ID
PKH2	854053

Other Ageing Factor Name
Dietary restriction

PKH2 deletion increases replicative lifespan by 20% in the alpha strain and by 15% in the a strain [

18340043]. Deletion of PKH2 increases chronological lifespan by 29% [

22319457] to 34% [

21447998] as well as by 19 - 54% (19, 24, 29, 54) in diploid cells [

21447998]. PKH2 mutation extends both replicative and chronological lifespan as well as cancels out DR-induced replicative and chronological lifespan extension [

21584246]. Mean and maximum replicative lifespan on AL is extended by 38 and 69%, respectively.

114

OB_008027

Gene Symbol	NCBI Gene ID
PNC1	852846

Other Ageing Factor Name
Dietary restriction

Cells with 5 copies of PNC1 have a 70% longer replicative lifespan which is cancelled out by SIR2 deletion. PNC1 is upregulated under glucose DR [

12736687]. Pnc1 reduces cellular nicotinamide levels, a product and noncompetitive inhibitor of Sir2 deacetylation reaction. Overexpression of PNC1 suppresses the effect of exogenously added nicotinamide on Sir2-dependent silencing at HM loci, telomeres and rDNA loci [12736687; 14729974]. Pnc1 catalyses the breakdown of nicotinamide to nicotinate and ammonia [

12736687]. Deletion of PNC1 shortens replicative lifespan approximately by 10% [

12736687] and largely prevents replicative lifespan extension of 0.5% glucose restriction. 0.5% glucose restriction slightly extends median replicative lifespan (by 10 - 15%) but not maximum replicative lifespan in pnc1Delta [

14724176]. PNC1 overexpression suppresses the inhibitory effect of exogenously added NAM on silencing, lifespan, and Hst1-mediated transcriptional repression [

14729974]. Increased expression of PNC1 is both necessary and sufficient for replicative lifespan extension by DR and low-intensity stress. Under non-stressing conditions (2% glucose, 30 degree Celsius), a strain with additional copies of PNC1 (5XPNC1) has 70% longer replicative lifespan than the wild-type and some cells live for more than 70 divisions. Neither DR nor heat stress further increase the lifespan of the 5XPNC1 strain [

12736687]. PNC1 deletion decreases chronological lifespan [

17110466].

12736687, 14724176, 14729974

115

OB_008028

Gene Symbol	NCBI Gene ID
rab-10	266836

Other Ageing Factor Name
Dietary restriction

rab-10 RNA interference significantly extends lifespan of wild-type by 14 - 16%, of daf-16 mutants by 47%, and of daf-2 by 46%, but fails to significantly further extend lifespan of eat-2 mutants. rab-10 RNAi does not affect pumping, but similar to DR reduces and delays reproduction as well as cause a slender appearance. rab-10 mRNA is 2-fold downregulated in response to DR [

16103914]. rab-10 RNAi significantly reduces paralysis in Q35YFP transgenic animals [

Gene Symbol	NCBI Gene ID
RAS2	855625

Other Ageing Factor Name
Dietary restriction

Overexpression of RAS2 causes a 43% increase in mean and 18% increase in maximum lifespan as well as postpones the age-related increase in generation time. RAS2 deletion causes a 23% decrease in mean and a 30% decrease in maximum lifespan [

8034612]. Deletion of RAS2 leads to a longer chronological lifespan [

21076178]. Deletion of the RAS2 gene, which functions upstream of CYR1, doubles the mean chronological lifespan by a mechanism that requires Msn2/4 and Sod2 [

12586694]. DR further extends chronological lifespan of ras2Delta [

Gene Symbol	NCBI Gene ID
RCR2	851566

Other Ageing Factor Name
Dietary restriction

RCR2 deletion extends mean replicative lifespan by 18% and cancels out the lifespan extending effect of DR [Wuttke et al., unpublished].

118

OB_008031

Gene Symbol	NCBI Gene ID
rheb-1	176327

Other Ageing Factor Name
Dietary restriction

rheb-1 RNAi extends lifespan by mimicking the DR effect. Under AL condition, rheb-1 RNAi extends lifespan by 19.1% and the longevity-promoting effects of two DR regimens sDR and intermittent fasting are abolished [

19079239]. Knockdown of rheb-1 by RNAi only during the adulthood increases mean, median and 75th %ile lifespan by 18-25, 25 and 23-24%, respectively, but failed so in skn-1 or daf-16 mutant (with and without FUdR). Knockdown of rheb-1 dramatically enhances stress tolerance in an skn-1, but not daf-16-dependent manner [

Gene Symbol	NCBI Gene ID
RIM15	850511

Other Ageing Factor Name
Dietary restriction

RIM15 deletion results in 50% reduction of maximal chronological lifespan [

11292860] and consistently decreases chronological lifespan under AL [

21076178]. Rim15 is required for chronological lifespan extension caused by deficiency in RAS2, TOR1, or SCH9, as well as by 0.5% glucose restriction, but not by water starvation [

Gene Symbol	NCBI Gene ID
Rpd3	38565

Other Ageing Factor Name
Dietary restriction

Males heterozygous for hypomorphic (partial loss-of-function) or null mutation of Rpd3 have a lifespan extension of 33% and 41 - 47%, respectively. Females heterozygous for a hypomorphic allele have a 52% increase in lifespan, but females carrying a null mutation have only modest increase in maximum lifespan (but not median lifespan). Longevity increases to the same extent in wild-type under low-calorie diet and rpd3 mutants fed normal diet. DR fails to further increase lifespan of rpd3 mutants. DR leads to a moderate but significant down-regulation of Rpd3, analogous to decrease obtained in heterozygotes carrying rpd3 mutation. rpd3 mutants fed normal food and wild-type fed low-calorie increase Sir2 expression two-fold [

Gene Symbol	NCBI Gene ID
RPD3	855386

Other Ageing Factor Name
Dietary restriction

Deletion of the histone deacetylase gene RPD3 extends lifespan by 41%, independently of an intact Sir silencing complex (in the short lived YSK661 strain) [

10512855]. Deletion of RPD3 extends replicative lifespan and there was no additive effect by neither 0.1% glucose nor amino acid restriction [

12213553]. RPD3 deletion increases rDNA silencing in a partially SIR2-dependent manner [

10082585]. Its effects on chromatin functional state were evidenced by enhanced silencing at the three known heterochromatic regions in the genome, the silent mating type (HM), subtelomeric, and rDNA loci, which occurred even in the absence of SIR3 [

Gene Symbol	NCBI Gene ID
RPL31A	851484

Other Ageing Factor Name
Dietary restriction

Deletion of RPL31A increases mean replicative lifespan by 45% [

16293764]. Mean replicative lifespan increases by 35% in the alpha strain and 50% in a strain [19030232; 18423200]. Mean replicative lifespan of the RPL31A deletion mutant increases by 35% in the ORF collection and by 29% in the remade strain [

22377630]. RPL31A deletion increases significantly replicative lifespan [

17174052]. Deletion of RPL31A extends replicative lifespan and is not further extended by 0.05% glucose restriction [

Gene Symbol	NCBI Gene ID
rpr	40015

Other Ageing Factor Name
Dietary restriction

Flies with ablated wings caused by overexpressing reaper (UAS-rpr) with a wing-sepcific Gal4 enhancer trap (1096-Gal4) exhibit only a 14% extension in lifespan compared to controls which exhibit a 61% extension upon DR [Katewa et al. 2012].

124

OB_008037

Gene Symbol	NCBI Gene ID
S6k	38654

Other Ageing Factor Name
Dietary restriction

Ubiquitous overexpression of a dominant-negative form of S6k (alias dS6K) increases mean lifespan by 22% and overexpression of a constitutively active form of S6k decreases mean lifespan by 34% at 29 degree Celsius. Overexpression of a dominant-negative form of S6k protects mutants from deleterious effects of rich food, as if mimicking the effect of DR [

Gene Symbol	NCBI Gene ID
sams-1	181370

Other Ageing Factor Name
Dietary restriction

sams-1 RNAi significantly extends lifespan of wild-type by 14 - 15%, of daf-16 mutant by 30% and daf-2 by 55%, but fails to significantly further extend lifespan of eat-2 mutants. mRNA level of sams-1 is 2-fold reduced in eat-2 mutants. Like DR, sams-1 RNAi reduces brood size and slightly reproductive timing as well as causes a slender phenotype [

16103914]. sams-1 RNAi significantly reduces paralysis in Q35YFP transgenic animals [

Gene Symbol	NCBI Gene ID
SCH9	856612

Other Ageing Factor Name
Dietary restriction

Transposon-mediated mutagenesis of SCH9, which encodes for a serine threonine kinase homologous to Akt/PKB, increases resistance to oxidants and thermal stress as well as extends chronological lifespan by 30%. SCH9 deletion increases chronological lifespan by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 are required for this life-extension. Deletion of the mitochondrial antioxidant enzyme superoxide dismutase gene SOD2 prevents the increased chronological lifespan caused by SCH9 deletion [

11292860]. Mutations that decrease the activity of the Ras/Cyr1/PKA pathway also extend longevity and increase stress resistance by activating transcription factors Msn2/Msn4 and Sod2 [

12855292]. SCH9 deletion mutants exhibit more than 3-fold extension of chronological lifespan. By day 9 of medium depletion all the wild-type cells were dead while 50% sch9 mutants survived [

17710147]. Deletion of SCH9 also increases resistance to heat shock and oxidative stress [

11292860], and increases replicative lifespan by 18% (in DBY746) [

12586694]. SCH9 deletion increases the replicative lifespan by 40% in the alpha strain [

18340043] and increases mean chronological lifespan by 97 - 246% (97, 133, 154, 226, 246) in diploid cells [

21447998]. Mutation or deletion of SCH9 increases resistance to oxidants and extends chronological lifespan [11292860; 16286010]. The extended lifespan of SCH9 deletion mutants is not further extended by low glucose DR and is independent of Sir2 [

16293764]. Deletion of RIM15 or GIS1 reverses chronological lifespan extension associated with sch9Delta. Water restriction further increases chronological lifespan of sch9Delta [

18225956]. Deletion of SCH9 results in a longer chronological lifespan [

21076178].

11292860, 16286010, 16293764, 18225956

127

OB_008040

Gene Symbol	NCBI Gene ID
SFA1	851386

Other Ageing Factor Name
Dietary restriction

sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Indicating that NO homeostasis is crucial during DR-induced replicative lifespan extension. Deleting YHB1 partially abolishes the DR-induced replicative lifespan extension, whereas deleting SFA1 alone had no effect. Yhb1 and Sfa1 may play redundant roles [

Gene Symbol	NCBI Gene ID
shk-1	174536

Other Ageing Factor Name
Dietary restriction

shk-1 encodes a shaker family of potassium channel which functions in muscle [

21059759], is expressed in sensory neurons [

16899454], and downregulated in space. Mutation or RNA interference of shk-1 extends lifespan on NGM agar covered with killed or live bacteria as well as in liquid culture medium. shk-1 RNAi extends mean, 75%ile, and maximum lifespan by 16, 15, and 22%. shk-1(RB1392) mutation extends mean, 75%ile, and maximum lifespan by 19-22, 19-21, and 20-24%. Lifespan extension by unc-17 mutation is totally abolished by RNAi inactivation of either daf-16 or skn-1. eat-2 RNAi shortens the lifespan of shk-1 mutants. RNAi inactivation of shk-1 reduces Q35 aggregation [

22768380]. Mutation and RNAi of shk-1 enhance pheromone-induced dauer formation [

22768380].

129

OB_008042

Gene Symbol	NCBI Gene ID
sir-2.1	177924

Other Ageing Factor Name
Dietary restriction

sir-2.1 deletion slightly reduces lifespan of wild-type [

16860373]. sir-2.1 overexpression extends lifespan by about 50% and this lifespan extension depends on DAF-16 activity as it is suppressed by mutation in daf-16 and it does not synergize with daf-2 [

11242085]. sir-2.1 suppresses longevity of unc-13 and eat-2, but not daf-2 or unc-64 mutants. sir-2.1 is therefore partially required for lifespan extension from mutation of eat-2 [

16860373], but is completely independent for lifespan extension from DR using a reduced feeding protocol [Kaeberlein et al. in press]. sDR increases lifespan of wild-type and sir-2.1 mutants to the same extent [

19239417]. Overrexpression of sir-2.1 synergizes with TGF-beta mutation (daf-4 and daf-1) for dauer formation [

11242085].

11242085, 16860373, 19239417

130

OB_008043

Gene Symbol	NCBI Gene ID
Sir2	34708

Other Ageing Factor Name
Dietary restriction

Overexpression of Sir2 (alias dSir2) extends lifespan by up to 57% and specifically median lifespan by 40-60%, whereas a decrease in Sir2 activity by mutation blocks the life-extending effect of caloric reduction or rpd3 mutations [

15520384]. rpd3 mutants fed normal food and wild-type fed a low-calorie diet increase dSir2 expression two-fold [

12459580]. Sir2 mutation does not reduce lifespan under AL. Ubiquitous Sir2 overexpression causes a 4-fold increase in Sir2 mRNA expression and an up to 57% increase in average lifespan (29% for females and 18% for males). A 10 - 20% increase in Sir2 mRNA levels causes no lifespan extension. High levels of Sir2 protein is found in nuclei of neurons and in nuclei and cytoplasm of fat body cells. Neuronal Sir2 overexpression extends average lifespan by 52% in females and 20% in males. Motor-neuronal specific expression fails to cause lifespan extension. Flies with no or with several decreased Sir2 gene function have no lifespan extension under DR. DR fails to cause further increase in lifespan or even reduces lifespan toward normal of Sir2 overexpression mutants. Mild Sir2 overexpression in the fat-body extends lifespan and reduces relative body fat content in both males and females [

Gene Symbol	NCBI Gene ID
SIR2	851520

Other Ageing Factor Name
Dietary restriction

Deletion of SIR2 shortens replicative lifespan by approximately 30%. Integration of a second copy of SIR2 into the wild-type strain leads to an extension of replicative lifespan by around 35% in W303R strain[

10521401]. Deletion of SIR2 causes genomic instability at rDNA array [

2647300] and shortens replicative lifespan by 50% [

11000115]. 0.5% glucose restriction fails to increase the short lifespan of sir2Delta [

11000115] probably duo to hyperaccumulations of extrachromosomal rDNA circles (ERCs) [

16311627]. 0.1% glucose restriction extends replicative lifespan of sir2 mutants [

12213553]. 0.5, 0.1 and 0.05% glucose restriction are able to increase lifespan of sir2;fob1 double mutant to a greater extent than in wild-type [

15328540]. 0.05% glucose restriction further extends replicative lifespan of SIR2 overexpression mutant [

15328540]. Sir2 blocks extreme chronological lifespan extension as the lack of Sir2 along with DR and/or mutations in the yeast AKT homolog, Sch9, or Ras pathways causes a dramatic chronological lifespan extension (6-fold) [

16286010]. Sir2 inhibits formation of ERCs and acts on histones as well metabolic enzymes among others. Overexpression extends replicative lifespan in several strains, but not in PSY316 [

15684413]. Chronological lifespan of sir2 deletion mutant is significantly extended compared with wild-type in water (extreme DR) but not in saturated cultures containing 2% glucose (ad libitum). SIR2 mutants are defective for telomere [

1913809] and HM silencing [6098447; 3297920]. have increased rDNA recombination [

2647300] and a loss of rDNA silencing [

9009206, 9009207].

2647300, 10521401, 11000115, 12213553, 15328540, 15684413, 16286010, 16311627

132

OB_008045

Gene Symbol	NCBI Gene ID
skn-1	177343

Other Ageing Factor Name
Dietary restriction

RNA interference of or mutations in skn-1 prevent the life-extension effects of dietary restriction [

17538612]. skn-1 transgenes that overexpress a constitutive nuclear form of SKN-1 in the intestine extend the mean lifespan by 5-21%, independently of DAF-16 [

18358814]. skn-1 mutation does not alter lifespan under AL, but cancels out the lifespan extension effect of lDR or food variation at all. Response to lDR in skn-1 mutant is restored by ectopic expression of skn-1 in ASI neurons and gut. Ectopic expression of skn-1b in ASI neurons rescued lDR longevity defects of skn-1. Ablation of ASI neurons completely suppresses the response to DR in wild-type or daf-16 mutants and cause a small increase in basal longevity of wild-type but not daf-16 mutants. lDR significantly increases SKN-1 expression in ASI neurons. lDR worms exhibit elevated respiration, which is absent in skn-1 mutants. skn-1 is necessary for increased respiration and the increase in respiration is necessary for lDR longevity effect, because two different inhibitors of mitochondrial electron transport chain complex III, myxothiazol and antimycin, suppress lDR longevity without shortening lifespan under AL. In contrast, the long life of a daf-2 mutant is not affected by antimycin. Some isoforms of SKN-1 are expressed from an operon downstream of bec-1. Beclin-1 mediates autophagy induced by nutrient deprivation. Therefore, skn-1 might be regulated by nutritional stress [

17538612]. IF significantly extends lifespan of skn-1 mutants [

19079239]. sDR extends lifespan of a skn-1 loss-of-function mutant (which displays a premature stop codon in all three isoforms) and wild-type to a similar extent [

19239417]. skn-1(zu67) mutation decreases mean, median, and maximum lifespan by 11-23, 13-28 and 12-23%, respectively, and totally cancels out lifespan extension by ragc-1 RNAi [

22560223].

17538612, 19079239, 19239417

133

OB_008046

Gene Symbol	NCBI Gene ID
slcf-1	186633

Other Ageing Factor Name
Dietary restriction

slcf-1 RNAi or mutation extends the lifespan. slcf-1 mutation increases average lifespan by 40%. DR (by dilution of bacteria on solid medium or by bacterial deprivation) failes to extend slcf-1 mutant's long lifespan and lifespan is even reduced by lowering bacteria concentration (i.e. higher strength of DR) [

Gene Symbol	NCBI Gene ID
SLM4	852369

Other Ageing Factor Name
Dietary restriction

SLM4 deletion blocks replicative lifespan extension by moderate DR [

Gene Symbol	NCBI Gene ID
smk-1	179243

Other Ageing Factor Name
Dietary restriction

RNA interference of smk-1 completely suppresses the extended longevity of daf-2 mutations and partly the life-extension of clk-1 mutants. smk-1 RNAi decreases mean and maximum lifespan of similar level than daf-16 knockdown [

16530049]. smk-1 RNAi only slightly shortens the lifespan of wild-type worms, but abolishes the extended lifespan of eat-2 mutants [

17476212]. Loss of smk-1 by temperature sensitive allele suppresses the extended lifespan under optimal bDR, but not the response to DR itself [

Gene Symbol	NCBI Gene ID
SRX1	853776

Other Ageing Factor Name
Dietary restriction

Extra copy of SRX1 counteracts age-related hyperoxidation of Tsa1 and extends replicative lifespan by 15 - 20% in a TSA1-dependent manner. Replicative lifespan extension in sir2;fob1 double mutant by DR is reduced by SRX1 deletion. Wild-type cells require SRX1 to fully extend lifespan. DR fails to further extend replicative lifespan of cells carrying an extra copy of SRX1. Mutation in CDC35 (adenylate cyclase), a genetic mimetic of DR, is dependent on SRX1 to extend replicative lifespan [

Gene Symbol	NCBI Gene ID
sty1	2541652

Other Ageing Factor Name
Dietary restriction

Deleting sty1 cancels out chronological lifespan extension and enhanced heat stress resistance by DR. Sty1 (phosphorylated) and Sty1-dependent gene transcription (atf1, gpx1, cta1, fbp1) is activated during DR in the stationary phase, but are barely activated in glucose rich medium [

Gene Symbol	NCBI Gene ID
SUR4	851087

Other Ageing Factor Name
Dietary restriction

Deletion of SUR4 cancels out replicative lifespan extension of 0.5% glucose DR [

Gene Symbol	NCBI Gene ID
TCO89	855922

Other Ageing Factor Name
Dietary restriction

TCO89 deletion increases chronological lifespan, increases mitochondrial oxygen consumption, but decreases mitochondrial and cellular ROS in early stationary phase [

21641548]. Deletion of TCO89 cancels out replicative lifespan extension by moderate DR [

Gene Symbol	NCBI Gene ID
Thor	33569

Other Ageing Factor Name
Dietary restriction

Null mutation in Thor (alias d4E-BP) causes a significant decrease in longevity (-25% median lifespan in males). Thor is strongly upregulated during starvation. foxo and Thor null mutants are compromised in stress resistant. Stress resistance of foxo null mutants is rescued by Thor overexpression [

16055649]. Thor is upregulated on the protein level in a foxo-independent manner upon DR, while it is transcriptional induced in a foxo-dependent fashion by starvation. Thor null mutants cancel out DR-induced lifespan extension, because mutants exhibit a diminished change in lifespan when nutrient conditions were varied. Ubiquitously expression of Thor rescued DR response in females and males. Thor null mutants have a wild-type similar reduction in egg production upon DR. Ubiquitously overexpression of wild-type Thor causes no change under AL, but an activated allele (with more than 3-fold increased binding activity to delF4E) significantly extends lifespan of females (weak allele) and females as well as males (strong allele). Mean lifespan is extended by 11 to 40%. Median lifespan of males and females is enhanced by by 11 and 22%, respectively. Maximum lifespan is extended by 16 and 18% for males and females, respectively. Under DR (0.25% YE) there is no lifespan extension, beyond the effect of DR alone, in all (wild-type, weak and strong) Thor alleles [

Gene Symbol	NCBI Gene ID
TOR1	853529

Other Ageing Factor Name
Dietary restriction

TOR1 deletion extends mean and maximum replicative lifespan by 21 and 25% [

16293764] as well as chronological lifespan [

21076178]. This lifespan extension is independent of SIR2 and additive with deletion of FOB1 [

16293764]. Deletion of TOR1 fails to increase the replicative lifespan of a sir2 mutant [

20947565]. Deletion of TOR1 substantially extends chronological lifespan, increasing median survival almost 3-fold (wild-type 4.5 days, tor1 null 12 days), i.e. by 167%. By 21 days in culture, the vast majority of wild-type cells had died (>99.9%), whereas many tor1 null cells remained viable. Deletion of TOR1 also extends the chronological lifespan of the relatively short-lived BY4742 strain, one of the two haploid genetic backgrounds of the widely used Yeast Knockout Collection available from Open Biosystems. Deletion of TOR1 fails to extend chronological lifespan in Petite strains that are unable to respire [

17403371]. TOR1 deletion increases replicative lifespan by 30% in the alpha strain and 20% in a strain [

19030232]. TOR1 deletion mutant have and increased mean and maximum replicative lifespan by 21% and 6%, respectively [

21931558]. Deletion of TOR1 extends replicative lifespan as well as chronological lifespan [

21076178] and glucose restriction fails to further extend the long replicative lifespan of tor1Delta [16293764; 16418483; 18225956]. Water starvation (extreme DR) further extends chronological lifespan of tor1 mutants [

18225956].

12124627, 14724176, 16293764, 16418483, 18225956

142

OB_008055

Gene Symbol	NCBI Gene ID
Tor	47396

Other Ageing Factor Name
Dietary restriction

Overexpression of the 11 kDa FKBP12-rapamycin binding domain increases mean lifespan by 20% and at 25 degree (15186745).

Gene Symbol	NCBI Gene ID
trx-1	181863

Other Ageing Factor Name
Dietary restriction

Thioredoxins regulate many cellular redox processes. trx-1 is mainly associated with neurons and is expressed in ASJ ciliated sensory neurons and to some extent also on the posterior-most internal cells. trx-1 reduces protein disulfides in the presence of a heterologous thioredoxin reductase. trx-1 null mutant display reduced mean and maximum lifespan [

16387300]. Mutants with a deletion in the trx-1 gene display a decrease in lifespan and are sensitive to oxidative stress [

16324156]. trx-1 overexpression extends lifespan in wild-type but not in eat-2 mutants. trx-1 deletion completely suppresses the lifespan extension caused by eat-2 mutation, but only partially suppresses that by daf-2 or osm-5 mutations. Ectopic expression of trx-1 in ASJ neurons (but not in the intestine) in trx-1 mutants rescues the lifespan-extension conferred by eat-2 mutation. trx-1 overexpression extends lifespan of wild-type but not in eat-2 mutants. trx-1 deletion almost completely suppresses lifespan extension induced by dietary deprivation (DD). DD upregulates trx-1 expression in ASJ neurons. DR activates trx-1 in ASJ neurons which in turn triggers a trx-1-dependent non-cell autonomous mechanism to extend adult lifespan [

21334311].

144

OB_008057

Gene Symbol	NCBI Gene ID
TSA1	854980

Other Ageing Factor Name
Dietary restriction

A gain-of-function allele of peroxiredoxin (thioredoxin peroxidase, Tsa1) causes a dominant oxidative stress-resistance and robust premature aging phenotype with reduced mean lifespan. These effect is not provoked by altered Tsa1 levels, nor can it be stimulated by deletion, haploinssufficiency or overexpression of wild-type allele [

20729566]. Disruption of TSA1 shortens chronological lifespan [

15129730]. Replicative lifespan extension by DR in sir2;fob1 double mutant is reduced by TSA1 deletion mutant. Wild-type cells require TSA1 to fully extend lifespan. Mutation in CDC35 (adenylate cyclase), a genetic mimetic of DR, is dependent on TSA1 to extend lifespan [

Gene Symbol	NCBI Gene ID
ubc-18	175985

Other Ageing Factor Name
Dietary restriction

ubc-18 overexpression is unable to extend lifespan (possibly, UBC-18 is not limiting for WWP-1 function in lifespan). Loss of ubc-18 function by mutation or RNAi reduces lifespan at 25 degree Celsius, but only slightly at 20 degree Celsius. RNAi depletion of ubc-18 completely suppresses increased longevity of eat-2 mutants. RNAi depletion of ubc-18 has no effect on long lifespan of isp-1 or daf-2 mutants. Combined knockdown of wwp-1 and ubc-18 by RNAi does not shorten lifespan any further than RNAi of either single gene. Knockdown of ubc-18 suppresses extended lifespan of wwp-1 overexpression [

Gene Symbol	NCBI Gene ID
ucp2	555812

Other Ageing Factor Name
Dietary restriction

Overexpression of ucp2 increases mean, median, and maximum lifespan by 42, 40, and 26%, which is non-additive with sDR [

Gene Symbol	NCBI Gene ID
unc-51	180311

Other Ageing Factor Name
Dietary restriction

unc-51(e369) mutation reduces mean but extends maximum lifespan. unc-51(e369) mutation reduces lifespan of eat-2(ad1116) mutants to that of wild-type [

18219227].

148

OB_008061

Gene Symbol	NCBI Gene ID
VAM7	852660

Other Ageing Factor Name
Dietary restriction

VAM7 deletion decreases replicative lifespan under AL and blocked DR-mediated lifespan extension. Replicative lifespan decreases by 70% on DR in VAM7 deletion mutant [

Gene Symbol	NCBI Gene ID
vps-34	172280

Other Ageing Factor Name
Dietary restriction

vps-34 RNAi does not significantly change lifespan of wild-type, but cancels out the lifespan extension effect of eat-2 mutation [

Gene Symbol	NCBI Gene ID
VPS20	855101

Other Ageing Factor Name
Dietary restriction

VPS20 deletion decreases mean and maximum replicative lifespan by 16% and 19%, respectively, and additionally cancels out the DR-induced replicative lifespan extension [Wuttke et al., unpublished].

151

OB_008064

Gene Symbol	NCBI Gene ID
VPS21	854256

Other Ageing Factor Name
Dietary restriction

Lack of VPS21 reduces lifespan under starvation conditions to a level similiar to that of wild-type cells incubated in synthetic complete medium and therefore blocked the lifespan-extending effect of DR [

Gene Symbol	NCBI Gene ID
VPS30	855983

Other Ageing Factor Name
Dietary restriction

VPS30 deletion prevents chronological lifespan extension induced by amino-acid DR [

Gene Symbol	NCBI Gene ID
VPS8	851261

Other Ageing Factor Name
Dietary restriction

Lack of VPS8 reduces lifespan under starvation conditions to a level similiar to that of wild-type cells incubated in synthetic complete medium and therefore blocked the lifespan-extending effect of DR [

Gene Symbol	NCBI Gene ID
wis1	2541055

Other Ageing Factor Name
Dietary restriction

Constitutive active mutation of wis1 extends chronological lifespan and there is no further beneficial effect of DR [

Gene Symbol	NCBI Gene ID
wwp-1	171647

Other Ageing Factor Name
Dietary restriction

RNA interference of wwp-1 decreases median lifespan by 9% in wild-type animals and 24% in daf-2 mutants [

18006689]. Loss of wwp-1 function by RNAi or mutation reduces lifespan at 25 degree Celsius, but not 20 degree Celsius. wwp-1 overexpression extends lifespan by up to 20%. Reduced levels of wwp-1 completely suppress the extended longevity of eat-2 mutants. Lifespan of wwp-1 mutants across entire food concentration range by bacterial dilution in liquid culture or on solid plates does not noticeable change. There is no difference in wwp-1 mRNA levels under AL and DR. RNAi reduction of pha-4, but not of daf-16 suppresses increased longevity by wwp-1 overexpression. Mutations in iron sulphur component of complex III, isp-1, increases longevity by reducing mitochondrial function. wwp-1 RNAi does not suppress the extended lifespan of isp-1 mutants and has only minor suppressive effects on lifespan of another mitochondrial mutant, clk-1, and in cyc-1 RNAi treated worms. RNAi depletion of wwp-1 has no effect on long lifespan of daf-2 mutants [

Gene Symbol	NCBI Gene ID
YDL180W	851374

Other Ageing Factor Name
Dietary restriction

YDL180W deletion impairs DR-mediated replicative lifespan extension [Wuttke et al., unpublished].

157

OB_008071

Gene Symbol	NCBI Gene ID
YPT7	855012

Other Ageing Factor Name
Dietary restriction

YPT7 deletion decreases replicative lifespan by 15% in the alpha strain [

18340043]. Deletion of YPT7 cancels out replicative lifespan extension of 0.5% glucose restriction and results under DR also into a shorter replicative lifespan than under AL [

Gene Symbol	NCBI Gene ID
YHB1	853149

Other Ageing Factor Name
Dietary restriction

sfa1;yhb1 double mutant cancels out the ability of moderate DR to extend replicative lifespan, but not chronological lifespan. Indicating that NO homeostasis during DR-induced replicative lifespan extension is crucial. Deleting YHB1 partially abolished DR-induced replicative lifespan extension, whereas deleting SFA1 alone had no effect. Yhb1 and Sfa1 may play redundant roles [

Data Type 2

Ageing Relevance:

yes (Exp. Analysis)
yes, but no ageing factor assigned (Exp. Analysis)
no (Exp. Analysis)
putative (Comp. Analysis)

Organism

Ageing Factor

Lifespan Observation

Reference

Observation Stable ID

Species

Strain

Gene

Compound

Other Ageing Factor

Significant Lifespan Effect

Change

Observed Lifespan

Reference Lifespan

Lifespan Unit

Measure

Temperature

Temperature Unit

Sex/Mating Type

Description

Author

Year

PubMed

DOI

Source

OB_004896

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing glucose from 2% to 0.02%.

increased

1370.59%

250

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4286)

OB_004905

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing glucose from 2% to 0.02%.

increased

1333.33%

258

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4295)

OB_004902

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing sodium acetate from 2% to 0.2%.

increased

733.33%

175

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4292)

OB_004901

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing sodium acetate from 2% to 0.02%.

increased

714.29%

171

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4291)

OB_004904

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing fructose from 2% to 0.02%.

increased

691.30%

182

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4294)

OB_004899

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing sodium acetate from 2% to 0.2%.

increased

672.22%

139

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4289)

OB_004900

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing sodium acetate from 2% to 0.02%.

increased

638.89%

133

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4290)

OB_004903

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing fructose from 2% to 0.2%.

increased

565.22%

153

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4293)

OB_004795

BY4743

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

500.00%

days

median

℃

matalpha

Addition of acetic acid as needed to maintain a concentration of 10 mM in solution prevented life span extension from transfer to water or dietary restriction.

Burtner et al.

2009

Lifespan Observations Database (ID: 2117)

OB_004813

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

500.00%

days

median

℃

matalpha

Reduced glucose (below 2%) increased chronological life span while increased glucose (more than 2%) shortened life span.

Murakami et al.

2008

Lifespan Observations Database (ID: 2158)

OB_004897

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing fructose from 2% to 0.02%.

increased

450.00%

132

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4287)

OB_004898

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing fructose from 2% to 0.2%.

increased

450.00%

132

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4288)

OB_004783

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

400.00%

days

median

℃

mata

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium.

Burtner et al.

2009

Lifespan Observations Database (ID: 2104)

OB_004793

BY4743

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

400.00%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium.

Burtner et al.

2009

Lifespan Observations Database (ID: 2114)

OB_004782

BY4743

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

380.00%

days

median

℃

diploid

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium.

Burtner et al.

2009

Lifespan Observations Database (ID: 2103)

OB_004784

PSY316AT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

340.00%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium.

Burtner et al.

2009

Lifespan Observations Database (ID: 2105)

OB_004785

DBY746

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

340.00%

days

median

℃

mata

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.05% glucose increases the chronological life span of cells grown in synthetic defined medium.

Burtner et al.

2009

Lifespan Observations Database (ID: 2106)

OB_000866

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

328.57%

days

median

℃

matalpha

Deletion of sir2, hst1, hst2, hst3, hst4 or all 5 sirtuins at the same time had no effect on chronological life span and did not reduce life span extension from dietary restriction at 0.5% or 0.05% glucose.

Murakami et al.

2008

Lifespan Observations Database (ID: 2171)

OB_000878

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

328.57%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2183)

OB_000879

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hst3	Saccharomyces cerevisiae	854190	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

328.57%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2184)

OB_004818

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

328.57%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2163)

OB_000872

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

275.00%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2177)

OB_000873

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

275.00%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2179)

OB_004823

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

275.00%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2178)

OB_000881

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
hst3	Saccharomyces cerevisiae	854190	deletion / null	-	unknown	unknown
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

257.14%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2187)

OB_004824

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

257.14%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2186)

OB_000859

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
osm-3	Caenorhabditis elegans	177141	unknown	p802	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

165.22%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2140)

OB_000860

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
tax-4	Caenorhabditis elegans	176297	unknown	p678	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

165.22%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2141)

OB_000854

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
che-11	Caenorhabditis elegans	179666	unknown	e1810	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

160.87%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2136)

OB_000867

PSY316AR

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

160.00%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2172)

OB_000868

PSY316AR

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

160.00%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2173)

OB_004819

PSY316AR

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

160.00%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2164)

OB_004820

PSY316AR

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

160.00%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2165)

OB_004916

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.
Dietary restriction	Nonessential amino acids removed from media.

increased

157.98%

30.7

11.9

divisions

mean

℃

mata

Combining two forms of dietary restriction, glucose restriction and nonessential amino acid elimination, increases life span by 158.0%

Jiang et al.

2002

Lifespan Observations Database (ID: 4462)

OB_003086

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RPD3	Saccharomyces cerevisiae	855386	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

142.86%

28.9

11.9

divisions

mean

℃

mata

Combining deletion of the histone deacetylase RPD3 with dietary restriction by removal of nonessential amino acids from the media increases lifespan by 142.9%, an extension similar to that imparted by amino acid restriction alone.

Jiang et al.

2002

Lifespan Observations Database (ID: 4461)

OB_000869

W303AR5

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

140.00%

days

median

℃

mata

Murakami et al.

2008

Lifespan Observations Database (ID: 2174)

OB_000870

W303AR5

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

140.00%

days

median

℃

mata

Murakami et al.

2008

Lifespan Observations Database (ID: 2175)

OB_004821

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

140.00%

days

median

℃

mata

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2166)

OB_004822

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.05%

increased

140.00%

days

median

℃

mata

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2167)

OB_004915

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

139.50%

28.5

11.9

divisions

mean

℃

mata

Dietary restriction by removal of nonessential amino acids from the media extends lifespan by 139.5%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4460)

OB_000851

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
che-3	Caenorhabditis elegans	172593	unknown	p801	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

139.13%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2132)

OB_000855

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-10	Caenorhabditis elegans	184883	unknown	e1387	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

139.13%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2137)

OB_004740

PSY316AUT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose
rho0	Absence of mitochondrial DNA.

increased

133.33%

divisions

mean

℃

matalpha

Dietary restriction at either 0.5% or 0.05% glucose increases the replicative life span of rho0 cells in the PSY316AUT strain background, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1115)

OB_005017

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 0.5%.

increased

130.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 0.5% significantly increase yeast chronological life span.

Goldberg et al.

2010

Lifespan Observations Database (ID: 931)

OB_000865

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

128.57%

days

median

℃

matalpha

Murakami et al.

2008

Lifespan Observations Database (ID: 2170)

OB_004817

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

128.57%

days

median

℃

matalpha

Dietary restriction by reducing the glucose concentration of the medium from 2% to 0.5% or 0.05% increased chronological life span in the BY4742, PSY316AR, and W303AR5 strain backgrounds.

Murakami et al.

2008

Lifespan Observations Database (ID: 2162)

OB_000092

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SCH9	Saccharomyces cerevisiae	856612	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

116.18%

52.1

24.1

divisions

mean

℃

matalpha

Deletion of TOR or SCH9 increase life span additively with deletion of FOB1 but not with dietary restriction. These data support the model that TOR, SCH9 and dietary restriction act in a single pathway that is distinct from the pathway that FOB1 acts in. Deletion of FOB1 is believed to increase replicative life span by reducing the accumulation of extrachromosomal rDNA circles (ERCs) in the mother cell [10207108].

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1091)

OB_004924

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

114.29%

divisions

mean

℃

mata

Dietary restriction by removal of nonessential amino acids from the media extends lifespan by 114.3%.

Wang et al.

2010

Lifespan Observations Database (ID: 4513)

OB_004895

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing glucose from 2% to 0.2%.

increased

111.77%

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4285)

OB_001666

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RTG3	Saccharomyces cerevisiae	852171	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose on synthetic defined medium

increased

109.09%

divisions

median

℃

mata

Deletion of RTG3 increased life span on synthetic defined medium and did not prevent life span extension from dietary restriction by reduced glucose availability.

Jiang et al.

2000

Lifespan Observations Database (ID: 29)

OB_003089

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HDA1	Saccharomyces cerevisiae	855710	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

106.77%

27.5

13.3

divisions

mean

℃

mata

Combining deletion of the histone deacetylase HDA1 with dietary restriction by reducing media glucose from 2% to 0.1% increases lifespan by 106.8%, which is greater than the increase observed from reducing glucose alone (59.4%) despite the inability of HDA1 deletion alone to alter lifespan.

Jiang et al.

2002

Lifespan Observations Database (ID: 4466)

OB_003090

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HDA1	Saccharomyces cerevisiae	855710	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

106.77%

27.5

13.3

divisions

mean

℃

mata

Combining deletion of the histone deacetylase HDA1 with dietary restriction by removal of nonessential amino acids from the media increases lifespan by 106.8%, an extension not significantly different from that imparted by amino acid restriction alone.

Jiang et al.

2002

Lifespan Observations Database (ID: 4468)

OB_004921

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

102.29%

26.5

13.1

divisions

mean

℃

mata

Dietary restriction byremoval of nonessential amino acids from the media extends replicative lifespan by 102.3%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4474)

OB_003092

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HDA1	Saccharomyces cerevisiae	855710	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.
Dietary restriction	Nonessential amino acids removed from media.

increased

102.26%

26.9

13.3

divisions

mean

℃

mata

Combining deletion of the histone deacetylase HDA1 with two forms of dietary restriction, glucose restriction and nonessential amino acid elimination, increases life span by 102.3%, an extension not significantly different from that imparted by the combined dietary restriction in the presence of HDA1.

Jiang et al.

2002

Lifespan Observations Database (ID: 4470)

OB_004919

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.
Dietary restriction	Nonessential amino acids removed from media.

increased

98.50%

26.4

13.3

divisions

mean

℃

mata

Combining two forms of dietary restriction, glucose restriction and nonessential amino acid elimination, increases life span by 98.5%

Jiang et al.

2002

Lifespan Observations Database (ID: 4469)

OB_003084

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RPD3	Saccharomyces cerevisiae	855386	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

97.48%

23.5

11.9

divisions

mean

℃

mata

Combining deletion of the histone deacetylase RPD3 with dietary restriction by reducing media glucose from 2% to 0.1% increases lifespan to a similar degree as dietary restriction alone, but greater than RPD3 alone.

Jiang et al.

2002

Lifespan Observations Database (ID: 4459)

OB_004918

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

83.46%

24.4

13.3

divisions

mean

℃

mata

Dietary restriction by removal of nonessential amino acids from the media extends lifespan by 83.5%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4467)

OB_004739

PSY316AUT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose
rho0	Absence of mitochondrial DNA.

increased

83.33%

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1114)

OB_004762

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose on synthetic defined medium

increased

81.82%

divisions

median

℃

mata

Deletion of RTG3 increased life span on synthetic defined medium and did not prevent life span extension from dietary restriction by reduced glucose availability.

Jiang et al.

2000

Lifespan Observations Database (ID: 1872)

OB_005018

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 0.1%.

increased

80.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 0.1% significantly increase yeast chronological life span.

Goldberg et al.

2010

Lifespan Observations Database (ID: 932)

OB_003087

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RPD3	Saccharomyces cerevisiae	855386	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.
Dietary restriction	Nonessential amino acids removed from media.

increased

78.99%

21.3

11.9

divisions

mean

℃

mata

Combining deletion of the histone deacetylase RPD3 with two forms of dietary restriction, glucose restriction and nonessential amino acid elimination, increases life span by 79.0%, which is substantially lower than the 158% extension observed from the two forms of dietary restriction in the presence of RPD3.

Jiang et al.

2002

Lifespan Observations Database (ID: 4463)

OB_004861

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^8 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source.

increased

78.03%

30.8

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4019)

OB_000155

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

76.63%

46.1

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1167)

OB_000116

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.005% glucose

increased

76.52%

43.6

24.7

℃

matalpha

Dietary restriction at 0.5%, 0.05%, or 0.005% glucose significantly increases the replicative life span of sir2 fob1 hst1 hst2 quadruple mutant cells in the BY4742 strain background. These data demonstrate that SIR2, HST1, and HST2 are dispensable, both individually and in combination, for life span extension from dietary restriction.

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1126)

OB_000151

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

75.86%

45.9

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1163)

OB_004914

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

73.95%

20.7

11.9

divisions

mean

℃

mata

Dietary restriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 73.9%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4457)

OB_004862

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^7 cells/mL. A 1000-fold reduction from control. UV-killed E. coli food source.

increased

72.83%

29.9

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4020)

OB_000015

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
vhl-1	Caenorhabditis elegans	181321	deletion / null	ok161	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

72.20%

41.5

24.1

days

mean

℃

hermaphrodite

Deletion of vhl-1 increased life span additively with dietary restriction by bacterial deprivation. These data support the model that VHL-1 and dietary restriction act in parallel pathways to promote longevity.

Mehta et al.

2009

Lifespan Observations Database (ID: 1013)

OB_000115

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

70.45%

42.1

24.7

℃

matalpha

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1125)

OB_004804

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

69.76%

34.8

20.5

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased median life span when initiated at day 4, 8, 14, or 20 of adulthood.

Smith et al.

2008

Lifespan Observations Database (ID: 2147)

OB_004210

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

69.35%

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose of the media from 2% to 0.05% significantly increases the life span of sir2 fob1 double mutant cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 901)

OB_000016

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
vhl-1	Caenorhabditis elegans	181321	deletion / null	ok161	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

68.53%

39.1

23.2

days

mean

℃

hermaphrodite

Mehta et al.

2009

Lifespan Observations Database (ID: 1014)

OB_000144

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

68.20%

43.9

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1157)

OB_000161

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.005% glucose

increased

67.05%

43.6

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1172)

OB_000606

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RTG2	Saccharomyces cerevisiae	852640	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose on synthetic defined medium

increased

66.67%

divisions

median

℃

mata

Deletion of RTG2 shortened life span on synthetic defined medium but did not prevent life span extension from dietary restriction by reduced glucose availability.

Jiang et al.

2000

Lifespan Observations Database (ID: 1874)

OB_004797

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 2nd day of adulthood.

increased

66.67%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation increases the life span of wild type and daf-16 mutants, but fails to increase the life span of hsf-1 mutants.

Steinkraus et al.

2008

Lifespan Observations Database (ID: 2124)

OB_004812

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduced glucose in the medium to 0.5%

increased

66.67%

days

median

℃

matalpha

Reduced glucose (below 2%) increased chronological life span while increased glucose (more than 2%) shortened life span.

Murakami et al.

2008

Lifespan Observations Database (ID: 2157)

OB_004211

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose

increased

64.52%

40.8

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose of the media from 2% to 0.1% significantly increases the life span of sir2 fob1 double mutant cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 902)

OB_004923

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

64.29%

divisions

mean

℃

mata

Dietary restriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 64.3%.

Wang et al.

2010

Lifespan Observations Database (ID: 4512)

OB_004801

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 8th day of adulthood.

increased

63.56%

38.6

23.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased median life span when initiated at day 4, 8, 14, or 20 of adulthood.

Smith et al.

2008

Lifespan Observations Database (ID: 2144)

OB_002738

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-16	Caenorhabditis elegans	172981	deletion / null	mu86	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^8 cells/mL. A 1000-fold reduction from control. UV-killed E. coli food source.

increased

63.01%

28.2

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4032)

OB_004215

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	over-expression	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

62.07%

42.3

26.1

divisions

mean

℃

matalpha

Overexpression of SIR2 combined with dietary restriction by reducing the glucose in the media from 2% to 0.05% significantly increased the life span of BY4742 mother cells beyond that of cells overexpressing SIR2 or cells subjected to dietary restriction alone.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 906)

OB_004802

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 14th day of adulthood.

increased

61.44%

38.1

23.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased median life span when initiated at day 4, 8, 14, or 20 of adulthood.

Smith et al.

2008

Lifespan Observations Database (ID: 2145)

OB_000160

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

61.30%

42.1

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1171)

OB_000110

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

61.17%

27.3

divisions

mean

℃

matalpha

Addition of 5 mM nicotinamide has no effect on replicative life span in BY4742 sir2 fob1 double mutant cells. Dietary restriction is still able to increase life span of sir2 fob1 double mutant cells treated with 5 mM nicotinamide. These data demonstrate that SIR2 and other nicotinamide-sensitive sirtuins are not required for life span extension from dietary restriction in the BY4742 strain background.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1120)

OB_004799

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

60.87%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of long-lived mutants defective for chemosensary function.

Smith et al.

2008

Lifespan Observations Database (ID: 2133)

OB_002739

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-16	Caenorhabditis elegans	172981	deletion / null	mu86	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^7 cells/mL. A 10000-fold reduction from control. UV-killed E. coli food source.

increased

59.54%

27.6

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4033)

OB_004917

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

59.40%

21.2

13.3

divisions

mean

℃

mata

Dietary restriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 59.4%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4464)

OB_004800

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 4th day of adulthood.

increased

59.32%

37.6

23.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased median life span when initiated at day 4, 8, 14, or 20 of adulthood.

Smith et al.

2008

Lifespan Observations Database (ID: 2143)

OB_004299

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

59.01%

35.3

22.2

days

mean

℃

hermaphrodite

Deletion of hif-1 failed to prevent life span extension from dietary restriction by bacterial deprivation. These data support the model that dietary restriction increases life span by a mechanism that does not require HIF-1.

Mehta et al.

2009

Lifespan Observations Database (ID: 993)

OB_005020

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

58.56%

35.2

22.2

days

mean

℃

hermaphrodite

Bacterial deprivation initiated at the second day of adulthood significantly increased life span.

Mehta et al.

2009

Lifespan Observations Database (ID: 963)

OB_000145

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.005% glucose

increased

57.09%

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1158)

OB_004906

Wa32

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing glucose from 2% to 0.2%.

increased

55.56%

days

mean

Dietary restriction by reducing availability of the carbon source increases life span in P. anserina.

van Diepeningen et al.

2010

Lifespan Observations Database (ID: 4296)

OB_000150

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

53.64%

40.1

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1162)

OB_004763

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose on synthetic defined medium

increased

53.33%

divisions

median

℃

mata

Deletion of RTG2 shortened life span on synthetic defined medium but did not prevent life span extension from dietary restriction by reduced glucose availability.

Jiang et al.

2000

Lifespan Observations Database (ID: 1873)

OB_004738

PSY316AUT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

52.36%

32.3

21.2

divisions

mean

℃

matalpha

Deletion of CYT1 does not significantly influence replicative life span in the PSY316AUT strain background. Dietary restriction still increases the life span of CYT1 deletion mutants, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction in yeast.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1108)

OB_004768

PS2025

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

51.60%

42.6

28.1

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1953)

OB_000027

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown
vhl-1	Caenorhabditis elegans	181321	deletion / null	ok161	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

51.60%

33.2

21.9

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased the life span of vhl-1(ok161); hif-1(ia04) double mutant animals. These data support the model that dietary restriction acts in a longevity pathway parallel to VHL-1/HIF-1.

Mehta et al.

2009

Lifespan Observations Database (ID: 1024)

OB_004798

Q35YFP

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 2nd day of adulthood.

increased

50.00%

days

median

℃

hermaphrodite

RNAi knock-down of hsf-1 blocked life span extension from dietary restriction by bacterial deprivation in Q35YPF animals expressing a 35 residue polyglutamine peptide.

Steinkraus et al.

2008

Lifespan Observations Database (ID: 2131)

100

OB_004992

gpa-9XS

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

50.00%

days

mean

Mean lifespan of gpa-9XS on low density feeding (when feeding E. coli DH5alpha) was approximately 50% longer than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5168)

101

OB_004803

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 20th day of adulthood.

increased

49.58%

35.3

23.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation significantly increased median life span when initiated at day 4, 8, 14, or 20 of adulthood.

Smith et al.

2008

Lifespan Observations Database (ID: 2146)

102

OB_000028

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown
vhl-1	Caenorhabditis elegans	181321	deletion / null	ok161	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

49.38%

24.1

days

mean

℃

hermaphrodite

Mehta et al.

2009

Lifespan Observations Database (ID: 1025)

103

OB_005019

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

47.30%

35.5

24.1

days

mean

℃

hermaphrodite

Bacterial deprivation initiated at the second day of adulthood significantly increased life span.

Mehta et al.

2009

Lifespan Observations Database (ID: 962)

104

OB_004769

PX176

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

46.84%

34.8

23.7

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1954)

105

OB_004298

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

46.06%

35.2

24.1

days

mean

℃

hermaphrodite

Mehta et al.

2009

Lifespan Observations Database (ID: 992)

106

OB_004860

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source.

increased

45.09%

25.1

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4018)

107

OB_000105

PSY316AUT

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
CYT1	Saccharomyces cerevisiae	854231	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

45.00%

divisions

mean

℃

matalpha

Dietary restriction at either 0.5% or 0.05% glucose increases the replicative life span of CYT1 deletion mutants in the PSY316AUT strain background, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1113)

108

OB_003138

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HAP4	Saccharomyces cerevisiae	853751	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

increased

42.86%

divisions

mean

℃

mata

Combining deletion of HAP4 with dietary retriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 42.9%. This extension is significantly shorter than the extension from dietary restriction alone, indicating that deletion of HAP4 partially suppresses replicative lifespan extension by amino acid restriction.

Wang et al.

2010

Lifespan Observations Database (ID: 4516)

109

OB_004737

PSY316AUT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

42.79%

31.7

22.2

divisions

mean

℃

matalpha

The replicative life span of rho0 cells is significantly shorter than wild type mother cells in the PSY316AUT strain background. Dietary restriction still increases the life span of PSY316AUT rho0 cells, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction in yeast.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1106)

110

OB_004771

Caenorhabditis remanei

EM464

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

40.90%

62.7

44.5

days

mean

℃

female

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1956)

111

OB_004991

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

40.74%

13.5

days

mean

Mean lifespan of N2 on low density feeding (when feeding E. coli DH5alpha) was approximately 41% longer than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5167)

112

OB_004827

UM-HET3

Other Ageing Factor Name	Additional Information
Dietary restriction	66%70% of the average amount eaten by ad-lib-fed mice of the same sex and age

increased

39.83%

1169

836

days

mean

female

A heterogeneous stock of mice, UM-HET3 (the first generation offspring of CByB6F1/J and C3D2F1/J parents) were used to test effects of six antiaging treatments on life span. In the first report of diet restriction in a structured, segregating heterogeneous population, essentially the same increases in mean and maximum life span as found in CByB6F1/J hybrid positive controls were observed. The results of treatment with N-acetyl-L-cysteine started at 7 months, and aspirin, nitroflurbiprofen, 4-hydroxy phenyl N-tert-butyl nitrone, and nordihydroguaiaretic acid, all started at 16-18 months are also reported. Only male UM-HET3 mice receiving N-acetyl-L-cysteine had significantly increased life span, and this may have been due to treatment-related inadvertent diet restriction. The other agents had no significant effects on life span. The use of UM-HET3 mice helps assure that these results are not the result of unresponsiveness of a single genotype but that they more broadly represent laboratory mice.

Flurkey et al.

2010

Lifespan Observations Database (ID: 2268)

113

OB_004766

MY2

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

38.31%

36.1

26.1

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1951)

114

OB_004829

CByB6F1

Other Ageing Factor Name	Additional Information
Dietary restriction	66%70% of the average amount eaten by ad-lib-fed mice of the same sex and age

increased

37.54%

1154

839

days

mean

male

Flurkey et al.

2010

Lifespan Observations Database (ID: 2270)

115

OB_000099

PSY316AUT

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
CYT1	Saccharomyces cerevisiae	854231	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

35.85%

28.8

21.2

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1107)

116

OB_002728

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^8 cells/mL. A 1000-fold reduction from control. UV-killed E. coli food source.

increased

35.84%

23.5

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4022)

117

OB_005008

Aedes aegypti

Other Ageing Factor Name	Additional Information
Dietary restriction	-

increased

35.00%

days

mean

A. aegypti females fed either single or no blood meals survived 30-40% longer than those given weekly blood meals. However, mosquitoes given weekly blood meals produced far more eggs.

Joy et al.

2010

20451597

doi:10.1016/j.exger.2010.04.009

Lifespan Observations Database (ID: 888)

118

OB_003368

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 0.1 X SY food solution.

increased

33.33%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 33% greater than controls on 0.1X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4790)

119

OB_004207

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

32.66%

32.9

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose of the media from 2% to 0.5% significantly increases the life span of sir2 fob1 double mutant cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 897)

120

OB_004828

UM-HET3

Other Ageing Factor Name	Additional Information
Dietary restriction	66%70% of the average amount eaten by ad-lib-fed mice of the same sex and age

increased

32.49%

1101

831

days

mean

male

Flurkey et al.

2010

Lifespan Observations Database (ID: 2269)

121

OB_004920

YPK9

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

32.00%

16.5

12.5

divisions

mean

℃

mata

Dietary restriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 32.0%.

Jiang et al.

2002

Lifespan Observations Database (ID: 4471)

122

OB_000148

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Compound Name	Quantity	Quantity Unit
Nicotinamide	5	millimolar

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

31.03%

34.2

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1160)

123

OB_005016

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 1%.

increased

30.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 1% significantly increase yeast chronological life span.

Goldberg et al.

2010

Lifespan Observations Database (ID: 930)

124

OB_000029

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown
vhl-1	Caenorhabditis elegans	181321	deletion / null	ok161	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

29.95%

28.2

21.7

days

mean

℃

hermaphrodite

RNAi knock-down of daf-2 significantly increased the life span of vhl-1(ok161); hif-1(ia04) double mutant animals. These data support the model that insulin-like signaling acts in a longevity pathway parallel to VHL-1/HIF-1.

Mehta et al.

2009

Lifespan Observations Database (ID: 1026)

125

OB_000163

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

29.89%

33.9

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1174)

126

OB_004767

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

29.80%

31.8

24.5

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1952)

127

OB_004765

JU262

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

29.72%

37.1

28.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1950)

128

OB_004733

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose
rho0	Absence of mitochondrial DNA.

increased

29.49%

30.3

23.4

divisions

mean

℃

matalpha

The replicative life span of rho0 cells does not differ significantly from that of wild type mother cells in the BY4742 strain background. Dietary restriction still increases the life span of BY4742 rho0 cells, demonstrating that mitochondrial respiration is not required for life span extension from dietary restriction in yeast.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1102)

129

OB_004734

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

29.49%

30.3

23.4

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1103)

130

OB_005011

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

29.44%

32.1

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose content of the media from 2% to 0.05% increased replicative life span of BY4742 mother cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 900)

131

OB_000537

Lifespan Observations Database (ID: 1798)

PSY316

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
CDC25	Saccharomyces cerevisiae	851019	non-null recessive	cdc25-10	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

29.25%

27.4

21.2

divisions

mean

℃

matalpha

The temperature sensitive cdc25-10 allele or dietary restriction alone increase life span, but combining them does not further increase life span, supporting the model that they act in the same pathway to modulate replicative life span.

Lin et al.

2000

11000115

132

OB_002727

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^7 cells/mL. A 1000-fold reduction from control. UV-killed E. coli food source.

increased

28.32%

22.2

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4021)

133

OB_004830

CByB6F1

Other Ageing Factor Name	Additional Information
Dietary restriction	66%70% of the average amount eaten by ad-lib-fed mice of the same sex and age

increased

28.17%

1101

859

days

mean

female

Flurkey et al.

2010

Lifespan Observations Database (ID: 2271)

134

OB_000114

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

26.72%

31.3

24.7

℃

matalpha

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1124)

135

OB_002737

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-16	Caenorhabditis elegans	172981	deletion / null	mu86	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source.

increased

26.59%

21.9

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4031)

136

OB_004996

doi:10.1371/journal.pgen.1002253

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

26.05%

27.1

21.5

divisions

mean

℃

matalpha

Replicative life span on DR and YPD plates. DR was at 0.05% glucose. 60 cells on each condition.

Kruegel et al.

2011

21931558

Lifespan Observations Database (ID: 5220)

137

OB_004865

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source. With FUDR.

increased

25.43%

21.7

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4039)

138

OB_000108

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Compound Name	Quantity	Quantity Unit
Nicotinamide	5	millimolar

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

25.27%

34.2

27.3

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1119)

139

OB_003395

Lifespan Observations Database (ID: 4818)

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
faah-1	Caenorhabditis elegans	177613	over-expression	rfls22	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	110^8c.f.u.ml1 E. coli, relative to (11010 colony-forming units (c.f.u.)ml1 Escherichia coli.

increased

25.00%

days

mean

℃

faah-1 overexpression extends lifespan approximately 25% in N2 wild-type animals under conditions of sub-optimal dietary restriction conditions.

Lucanic et al.

2011

21562563

doi:10.1038/nature10007

140

OB_004300

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

24.57%

28.9

23.2

days

mean

℃

hermaphrodite

Mehta et al.

2009

Lifespan Observations Database (ID: 994)

141

OB_004856

doi:10.1111/j.1474-9726.2010.00560.x

Outbred population caught in early October 2006 in a vineyard ne

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduction of dietary yeast from 12% to 5%

increased

24.24%

days

median

℃

female

Reducing dietary yeast from 12% to 5% or 2% extended life span. 1% yeast was detrimental for longevity.

Burger et al.

2010

20156204

Lifespan Observations Database (ID: 3971)

142

OB_004750

Lifespan Observations Database (ID: 1799)

PSY316

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

23.58%

26.2

21.2

divisions

mean

℃

matalpha

Lin et al.

2000

11000115

143

OB_004859

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^10 cells/mL. A 10-fold reduction from control. UV-killed E. coli food source.

increased

23.12%

21.3

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4017)

144

OB_004770

PX178

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

22.97%

36.4

29.6

days

mean

℃

hermaphrodite

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1955)

145

OB_005021

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

22.41%

28.4

23.2

days

mean

℃

hermaphrodite

Bacterial deprivation initiated at the second day of adulthood significantly increased life span.

Mehta et al.

2009

Lifespan Observations Database (ID: 964)

146

OB_000299

doi:10.1016/j.cell.2008.02.037

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	normal	-	unknown	unknown
GCN4	Saccharomyces cerevisiae	856709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

22.26%

34.6

28.3

divisions

mean

℃

matalpha

Deletion of GCN4 prevents maximal life span extension from deletion of TOR1, deletion of SCH9, or dietary restriction. From Supplemental Figure 6.

Steffen et al.

2008

18423200

Lifespan Observations Database (ID: 1301)

147

OB_004736

PSY316AUT

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose
rho0	Absence of mitochondrial DNA.

increased

22.07%

27.1

22.2

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1105)

148

OB_005012

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

21.84%

31.8

26.1

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose in the media from 2% to 0.05% significantly increased the life span of BY4742 mother cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 907)

149

OB_005010

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose

increased

21.37%

30.1

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose content of the media from 2% to 0.1% increased replicative life span of BY4742 mother cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 899)

150

OB_004730

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

20.85%

28.4

23.5

divisions

mean

℃

matalpha

Deletion of TOR1 significantly increased replicative life span of BY4742 cells but does not further increase life span when combined with dietary restriction (0.05% glucose). These data support the model that TOR1 and dietary restriction act in the same pathway to promote longevity.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1084)

151

OB_004982

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

increased

20.59%

19.56

16.22

days

mean

Mean lifespan of N2 worms on low density feeding (live OP50) was approximately 21% longer than control.

Hahm et al.

2011

Lifespan Observations Database (ID: 5156)

152

OB_000085

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
TOR1	Saccharomyces cerevisiae	853529	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

20.43%

28.3

23.5

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1083)

153

OB_002729

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source.

increased

20.23%

20.8

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4023)

154

OB_004301

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hif-1	Caenorhabditis elegans		deletion / null	ia04	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

increased

20.09%

26.3

21.9

days

mean

℃

hermaphrodite

Mehta et al.

2009

Lifespan Observations Database (ID: 995)

155

OB_004986

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

increased

20.00%

19.8

16.5

days

mean

Mean lifespan on low density feeding (when feeding dead OP50) was approximately 20% longer than on high-density diet.

Hahm et al.

2011

Lifespan Observations Database (ID: 5161)

156

OB_000159

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

19.92%

31.3

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1170)

157

OB_004745

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

19.54%

31.2

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1140)

158

OB_004983

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

increased

19.15%

23.5

days

max

Maximum lifespan of N2 worms on low density feeding (live OP50) was approximately 19% longer than control.

Hahm et al.

2011

Lifespan Observations Database (ID: 5157)

159

OB_004857

doi:10.1111/j.1474-9726.2010.00560.x

Outbred population caught in early October 2006 in a vineyard ne

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduction of dietary yeast from 12% to 2%

increased

18.18%

days

median

℃

female

Reducing dietary yeast from 12% to 5% or 2% extended life span. 1% yeast was detrimental for longevity.

Burger et al.

2010

20156204

Lifespan Observations Database (ID: 3972)

160

OB_004864

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source. With FUDR.

increased

17.88%

21.1

17.9

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4034)

161

OB_004987

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

17.79%

24.5

20.8

days

mean

Mean lifespan of of N2 on low density feeding (when feeding B. subtillis) was approximately 18% longer than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5163)

162

OB_000143

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

17.62%

30.7

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1156)

163

OB_004988

gpa-9XS

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

17.35%

19.6

days

mean

Mean lifespan of of gpa-9XS on low density feeding (when feeding B. subtillis) was approximately 17% longer than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5164)

164

OB_000847

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-16	Caenorhabditis elegans	172981	non-null recessive	mu86	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 2nd day of adulthood.

increased

16.67%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation increases the life span of wild type and daf-16 mutants, but fails to increase the life span of hsf-1 mutants.

Steinkraus et al.

2008

Lifespan Observations Database (ID: 2128)

165

OB_000086

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SCH9	Saccharomyces cerevisiae	856612	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

15.50%

29.8

25.8

divisions

mean

℃

matalpha

Deletion of SCH9 significantly increased replicative life span of BY4742 cells but does not further increase life span when combined with dietary restriction (0.05% glucose). These data support the model that SCH9 and dietary restriction act in the same pathway to promote longevity.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1086)

166

OB_000154

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
hst3	Saccharomyces cerevisiae	854190	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

15.33%

30.1

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1166)

167

OB_000104

PSY316AUT

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
CYT1	Saccharomyces cerevisiae	854231	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

15.00%

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1112)

168

OB_000103

PSY316AUT

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
CYT1	Saccharomyces cerevisiae	854231	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose
rho0	Absence of mitochondrial DNA.

increased

14.62%

24.3

21.2

divisions

mean

℃

matalpha

PSY316AUT rho0 cells also deleted for CYT1 are short-lived, but still have their life span extended by dietary restriction. These data demonstrate that mitochondrial respiration is not required for life span extension from dietary restriction in the PSY316AUT strain background.

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1111)

169

OB_003137

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
HAP4	Saccharomyces cerevisiae	853751	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

increased

14.29%

divisions

mean

℃

mata

Combining deletion of HAP4 with dietary retriction by reducing glucose from 2% to 0.1% extends replicative lifespan by 14.3%. This extension is significantly shorter than the extension from dietary restriction alone, indicating that deletion of HAP4 partially suppresses replicative lifespan extension by glucose restriction.

Wang et al.

2010

Lifespan Observations Database (ID: 4515)

170

OB_005009

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

14.11%

28.3

24.8

divisions

mean

℃

matalpha

Dietary restriction by reducing the glucose content of the media from 2% to 0.5% increased replicative life span of BY4742 mother cells.

Kaeberlein et al.

2004

Lifespan Observations Database (ID: 898)

171

OB_004993

gpa-9XS

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

13.95%

19.6

17.2

days

mean

Mean lifespan of gpa-9XS on low density feeding (when feeding E. coli BL21) was approximately 14% longer than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5169)

172

OB_004866

Nothobranchius furzeri

GRZ

Other Ageing Factor Name	Additional Information
Dietary restriction	Every other day feeding. Control fish followed a standard diet regime, that means they were fed normally twice a day with Chironomus larvae, while DR fish were fed twice a day every other day.

increased

13.04%

80.5

days

median

℃

Dietary restriction increased median and maximum life span in the laboratory GRZ strain of Nothobranchius furzeri. In a second strain, MZM-04/10P, dietary restriction reduced median life span but extended maximum life span due to a wave of early mortality in the dietary restricted animals.

Terzibasi et al.

2009

19302373

doi:10.1111/j.1474-9726.2009.00455.x

Lifespan Observations Database (ID: 4123)

173

OB_004731

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

12.79%

29.1

25.8

divisions

mean

℃

matalpha

Kaeberlein et al.

2005

Lifespan Observations Database (ID: 1085)

174

OB_004984

gpa-9

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

increased

12.64%

19.43

17.25

days

mean

Mean lifespan on low density feeding (when fed live OP50) was approximately 13% longer than on high-density diet.

Hahm et al.

2011

Lifespan Observations Database (ID: 5159)

175

OB_004994

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

12.50%

19.8

17.6

days

mean

Mean lifespan of N2 worms on low density feeding (when feeding E. coli BL21) was not significantly different than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5170)

176

OB_004985

gpa-9

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

increased

11.11%

days

max

Maximum lifespan on low density feeding (when fed live OP50) was approximately 11% longer than on high-density diet.

Hahm et al.

2011

Lifespan Observations Database (ID: 5160)

177

OB_004744

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

10.34%

28.8

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1139)

178

OB_003211

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-2	Caenorhabditis elegans	172632	deletion / null	gk257	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

increased

9.02%

26.6

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4621)

179

OB_004852

Rattus norvegicus

Fischer-344

Other Ageing Factor Name	Additional Information
Dietary restriction	30% reduction from ad lib control started at 6 months of age

increased

8.80%

866.5

796.4

male

Although CR significantly extended early life span (25th quantile) of Fischer-344 rats, metformin supplementation (300 mg/kg/day) did not significantly increase life span at any quantile (25th, 50th, 75th, or 90th), overall or maximum life span (p > .05) compared with control.

Smith et al.

2010

20304770

doi:10.1093/gerona/glq033

Lifespan Observations Database (ID: 3860)

180

OB_002736

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
daf-16	Caenorhabditis elegans	172981	deletion / null	mu86	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^10 cells/mL. A 10-fold reduction from control. UV-killed E. coli food source.

increased

6.94%

18.5

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4030)

181

OB_004990

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

increased

5.00%

12.6

days

mean

Mean lifespan of N2 on low density feeding (when feeding P. corrugate) was not significantly different than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5166)

182

OB_004989

gpa-9XS

Other Ageing Factor Name	Additional Information
Dietary restriction	low density feeding (LDF) conditions (OD = 0.18) relative to high density feeding (HDF) conditions (OD - 1.5).

none

4.17%

12.5

days

mean

Mean lifespan of of gpa-9XS on low density feeding (when feeding P. corrugate) was not significantly different than high-density feeding of same bacteria.

Hahm et al.

2011

Lifespan Observations Database (ID: 5165)

183

OB_002730

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^10 cells/mL. A 10-fold reduction from control. UV-killed E. coli food source.

none

4.05%

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4024)

184

OB_004772

Caenorhabditis remanei

SB146

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation

none

4.03%

38.7

37.2

days

mean

℃

female

Dietary restriction by bacterial deprivation increased life span in several wild-derived C. elegans species and in the related nematode C. remanei.

Sutphin et al.

2008

Lifespan Observations Database (ID: 1957)

185

OB_000165

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Compound Name	Quantity	Quantity Unit
Nicotinamide	5	millimolar

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

none

2.30%

26.7

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1176)

186

OB_003394

Lifespan Observations Database (ID: 4817)

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
faah-1	Caenorhabditis elegans	177613	over-expression	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1109c.f.u.ml1 E. coli relative to (11010 colony-forming units (c.f.u.)ml1 Escherichia coli.

none

0.00%

days

mean

℃

Lifespan is not significantly different between N2 and a faah-1 overexpressing line under conditions of optimal dietary restriction.

Lucanic et al.

2011

21562563

doi:10.1038/nature10007

187

OB_004330

BY4743

Compound Name	Quantity	Quantity Unit
Acetic acid	0	-

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

none

0.00%

days

median

℃

matalpha

Addition of acetic acid as needed to maintain a concentration of 10 mM in solution prevented life span extension from transfer to water or dietary restriction.

Burtner et al.

2009

Lifespan Observations Database (ID: 2118)

188

OB_003207

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-5	Caenorhabditis elegans	173776	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

none

-2.05%

23.9

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4618)

189

OB_003212

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-2	Caenorhabditis elegans	172632	deletion / null	ok1030	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

none

-2.05%

23.9

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4622)

190

OB_003649

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
gpa-9	Caenorhabditis elegans	191659	over-expression	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.18-OD diet (low density feeding; LDF) relative to 1.5-OD diet (high-density feeding; HDF)

none

-2.40%

20.3

20.8

days

mean

The gpa-9XS strain, carrying extra copies of the gpa-9 gene, showed no significant difference in MLS between LDF conditions and HDF conditions when fed live OP50.

Hahm et al.

2011

Lifespan Observations Database (ID: 5158)

191

OB_003213

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-1	Caenorhabditis elegans	174141	deletion / null	tm783	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

none

-2.87%

23.7

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4623)

192

OB_003214

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-1	Caenorhabditis elegans	174141	deletion / null	tm776	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

decreased

-6.15%

22.9

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4624)

193

OB_003210

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-3	Caenorhabditis elegans	181748	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

decreased

-6.56%

22.8

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4620)

194

OB_004867

Nothobranchius furzeri

MZM-04/10P

Other Ageing Factor Name	Additional Information
Dietary restriction	Every other day feeding. Control fish followed a standard diet regime, that means they were fed normally twice a day with Chironomus larvae, while DR fish were fed twice a day every other day.

decreased

-6.90%

189

203

days

median

℃

Terzibasi et al.

2009

19302373

doi:10.1111/j.1474-9726.2009.00455.x

Lifespan Observations Database (ID: 4124)

195

OB_003208

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sod-4	Caenorhabditis elegans	176336	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Adult worms were transferred to liquid axenic media (3% soy protein, 3% yeast extract, .05% hemogloblin (Houthoofd et al., 2002) and 5 mg/L of cholesterol) that was supplemented with tetracycline (12.5 mg/L) and ampicillin (100 mg/L) to prevent bacterial growth. The worms were transferred to fresh media approximately every 2 weeks.

decreased

-6.97%

22.7

24.4

days

mean

℃

Inactivation of sod isoforms had no significant effect on the increased lifespan from dietary restriction.

Yen et al.

2009

Lifespan Observations Database (ID: 4619)

196

OB_004743

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

decreased

-8.13%

22.6

24.6

℃

mata

Dietary restriction at 0.5%, 0.05%, or 0.005% glucose fails to increase replicative life span in the W303AR5 strain background.

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1132)

197

OB_002743

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source. With FUDR.

decreased

-9.25%

15.7

17.3

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4038)

198

OB_004746

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	0.005% glucose

decreased

-11.49%

23.1

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1141)

199

OB_000844

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hsf-1	Caenorhabditis elegans	173078	non-null recessive	sy441	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 2nd day of adulthood.

decreased

-12.50%

days

median

℃

hermaphrodite

Dietary restriction by bacterial deprivation increases the life span of wild type and daf-16 mutants, but fails to increase the life span of hsf-1 mutants.

Steinkraus et al.

2008

Lifespan Observations Database (ID: 2125)

200

OB_004815

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Amino acids reduced to 0.1X the normal concentration

decreased

-14.29%

days

median

℃

matalpha

Increased amino acid content (10X) of the synthetic defined medium increased chronological life span while reduced amino acid content had little effect.

Murakami et al.

2008

Lifespan Observations Database (ID: 2160)

201

OB_004816

BY4742

Other Ageing Factor Name	Additional Information
Dietary restriction	Amino acids reduced to 0.5X the normal concentration

decreased

-14.29%

days

median

℃

matalpha

Increased amino acid content (10X) of the synthetic defined medium increased chronological life span while reduced amino acid content had little effect.

Murakami et al.

2008

Lifespan Observations Database (ID: 2161)

202

OB_003096

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Nonessential amino acids removed from media.

decreased

-16.03%

13.1

divisions

mean

℃

mata

Combining deletion of the histone deacetylase SIR2 with dietary restriction by removal of nonessential amino acids from the media reduces lifespan by 16.0%. Deletion of SIR2 partially prevented lifespan extension from amino acid restriction.

Jiang et al.

2002

Lifespan Observations Database (ID: 4476)

203

OB_003705

doi:10.1371/journal.pgen.1002253

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
RPN4	Saccharomyces cerevisiae	851542	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

decreased

-16.28%

21.5

divisions

mean

℃

matalpha

Replicative life span on YEPD plates or 0.05% glucose plates. 80 cells of rpn4 DR, 60 cells of WT.

Kruegel et al.

2011

21931558

Lifespan Observations Database (ID: 5221)

204

OB_004239

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
pex5	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 1%.

decreased

-20.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 1% significantly decreased the chronological life span of cells deleted for PEX5, in contrast to increasing the life span of wild type cells.

Goldberg et al.

2010

Lifespan Observations Database (ID: 935)

205

OB_004742

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

decreased

-23.17%

18.9

24.6

℃

mata

Dietary restriction at 0.5%, 0.05%, or 0.005% glucose fails to increase replicative life span in the W303AR5 strain background.

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1131)

206

OB_003370

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 0.4 X SY food solution.

decreased

-23.81%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 24% shorter than controls on 0.4X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4792)

207

OB_003366

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 0 X SY food solution.

decreased

-25.00%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 25% shorter than controls.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4789)

208

OB_003094

YPK9

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Media glucose reduced from 2% to 0.1%.

decreased

-27.20%

9.1

12.5

divisions

mean

℃

mata

Combining deletion of the histone deacetylase SIR2 with dietary restriction by reducing media glucose from 2% to 0.1% reduces lifespan by 27.2%. Deletion of SIR2 did not prevent lifespan extension from glucose restriction.

Jiang et al.

2002

Lifespan Observations Database (ID: 4473)

209

OB_004858

doi:10.1111/j.1474-9726.2010.00560.x

Outbred population caught in early October 2006 in a vineyard ne

Other Ageing Factor Name	Additional Information
Dietary restriction	Reduction of dietary yeast from 12% to 1%

decreased

-31.82%

days

median

℃

female

Reducing dietary yeast from 12% to 5% or 2% extended life span. 1% yeast was detrimental for longevity.

Burger et al.

2010

20156204

Lifespan Observations Database (ID: 3973)

210

OB_003372

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 1.0X SY food solution.

decreased

-32.08%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 32% shorter than controls on 1.0X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4794)

211

OB_004741

W303AR5

Other Ageing Factor Name	Additional Information
Dietary restriction	0.005% glucose

decreased

-34.15%

16.2

24.6

℃

mata

Dietary restriction at 0.5%, 0.05%, or 0.005% glucose fails to increase replicative life span in the W303AR5 strain background.

Kaeberlein et al.

2006

Lifespan Observations Database (ID: 1130)

212

OB_002740

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
drr-2	Caenorhabditis elegans	259546	over-expression	EQ19	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	1 x 10^9 cells/mL. A 100-fold reduction from control. UV-killed E. coli food source. With FUDR.

decreased

-34.64%

11.7

17.9

days

mean

℃

hermaphrodite

Overexpression of DRR-2 has little effect on wild type life span but blunts life span extension from inhibition of TOR or from DR.

Ching et al.

2010

Lifespan Observations Database (ID: 4035)

213

OB_004521

Lifespan Observations Database (ID: 4825)

Compound Name	Quantity	Quantity Unit
EPEA (eicosapentaenoyl ethanolamide)	50	M

Other Ageing Factor Name	Additional Information
Dietary restriction	110^9c.f.u.ml1 E. coli, relative to (11010 colony-forming units (c.f.u.)ml1 Escherichia coli.

decreased

-37.04%

days

mean

℃

EPEA treatment completely suppresses the effect of optimal dietary restriction on wild-type N2 lifespan (110^9 c.f.u.ml1 E. coli.

Lucanic et al.

2011

21562563

doi:10.1038/nature10007

214

OB_003371

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 0.6X SY food solution.

decreased

-37.84%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 38% shorter than controls on 0.6X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4793)

215

OB_003369

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 0.2 X SY food solution.

decreased

-47.30%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 47% shorter than controls on 0.2X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4791)

216

OB_003373

dFOXO21a/+

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
foxo	Drosophila melanogaster	41709	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Food dilution series on SY food, from 0 to 1.4 X dilution. Dilution 1.4X SY food solution.

decreased

-50.00%

days

median

℃

female

Lifespan of dFOXO null transheterozygotes females was approximately 50% shorter than controls on 1.4X SY food dilution.

Giannakou et al.

2008

Lifespan Observations Database (ID: 4795)

217

OB_004238

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
pex5	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 0.5%.

decreased

-50.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 0.5% significantly decreased the chronological life span of cells deleted for PEX5, in contrast to increasing the life span of wild type cells.

Goldberg et al.

2010

Lifespan Observations Database (ID: 934)

218

OB_000848

Q35YFP

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hsf-1	Caenorhabditis elegans	173078	RNAi knockdown	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Bacterial deprivation beginning at the 2nd day of adulthood.

decreased

-53.85%

days

median

℃

hermaphrodite

RNAi knock-down of hsf-1 blocked life span extension from dietary restriction by bacterial deprivation in Q35YPF animals expressing a 35 residue polyglutamine peptide.

Steinkraus et al.

2008

Lifespan Observations Database (ID: 2129)

219

OB_004237

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
pex5	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose concentration of the medium from 2% to 0.1%.

decreased

-70.00%

days

mean

℃

matalpha

Reducing the glucose concentration of the synthetic defined culture medium from 2% to 0.1% significantly decreased the chronological life span of cells deleted for PEX5, in contrast to increasing the life span of wild type cells.

Goldberg et al.

2010

Lifespan Observations Database (ID: 933)

220

OB_000167

BY4742

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
FOB1	Saccharomyces cerevisiae	851688	deletion / null	-	unknown	unknown
HST1	Saccharomyces cerevisiae	854086	deletion / null	-	unknown	unknown
HST2	Saccharomyces cerevisiae	856092	deletion / null	-	unknown	unknown
hst3	Saccharomyces cerevisiae	854190	deletion / null	-	unknown	unknown
hst4	Saccharomyces cerevisiae	851772	deletion / null	-	unknown	unknown
SIR2	Saccharomyces cerevisiae	851520	deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

decreased

-75.10%

6.5

26.1

divisions

mean

℃

matalpha

Data from Table 1: Replicative lifespans of different sirtuin mutants in response to calorie restriction or nicotinamide.

Tsuchiya et al.

2006

Lifespan Observations Database (ID: 1178)

221

OB_001146

doi:10.1074/jbc.M110.175562

JY333

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
pma1	Schizosaccharomyces pombe	2542664	loss of function	L18	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

A partial loss of function mutation in Pma1 or treatment with the Pma1-inhibitory chemical vanadate resulted in increased chronological lifespan in Schizosaccharomyces pombe

Ito et al.

2010

20829365

Lifespan Observations Database (ID: 2463)

222

OB_002879

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
sir2	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose of the medium from 2% to 0.5%

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4167)

223

OB_002884

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hst1	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose of the medium from 2% to 0.5%

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4171)

224

OB_002886

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hst2	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose of the medium from 2% to 0.5%

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4173)

225

OB_002888

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hst3	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose of the medium from 2% to 0.5%

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4175)

226

OB_002893

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
gpa2	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological life span of gpa2 or hxk2 mutants.

Smith et al.

2007

Lifespan Observations Database (ID: 4199)

227

OB_002896

BY4741

Gene Symbol	Species	NCBI Gene ID	Allele Type	Allele Name	Reference Allele Type	Allele Change
hxk2	Saccharomyces cerevisiae		deletion / null	-	unknown	unknown

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological life span of gpa2 or hxk2 mutants.

Smith et al.

2007

Lifespan Observations Database (ID: 4200)

228

OB_004462

BY4741

Compound Name	Quantity	Quantity Unit
Sorbitol	1	M

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4190)

229

OB_004463

BY4741

Compound Name	Quantity	Quantity Unit
Sorbitol	2	M

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4191)

230

OB_004464

BY4741

Compound Name	Quantity	Quantity Unit
Sorbitol	0.5	M

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4192)

231

OB_004465

BY4741

Compound Name	Quantity	Quantity Unit
Sorbitol	0.1	M

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4193)

232

OB_004842

doi:10.1074/jbc.M110.175562

JY333

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

A partial loss of function mutation in Pma1 or treatment with the Pma1-inhibitory chemical vanadate resulted in increased chronological lifespan in Schizosaccharomyces pombe

Ito et al.

2010

20829365

Lifespan Observations Database (ID: 2464)

233

OB_004868

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	Reducing the glucose of the medium from 2% to 0.5%

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4165)

234

OB_004869

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	1% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4183)

235

OB_004870

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4184)

236

OB_004871

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	0.1% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4185)

237

OB_004872

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	0.05% glucose

increased

days

max

℃

mata

Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins.

Smith et al.

2007

Lifespan Observations Database (ID: 4186)

238

OB_004875

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction by reduced glucose increases life span at 37C and 23C

Smith et al.

2007

Lifespan Observations Database (ID: 4196)

239

OB_004876

BY4741

Other Ageing Factor Name	Additional Information
Dietary restriction	0.5% glucose

increased

days

max

℃

mata

Calorie restriction by reduced glucose increases life span at 37C and 23C

Smith et al.

2007

Lifespan Observations Database (ID: 4197)

240

OB_004959

Ceratitis capitata

Other Ageing Factor Name

Additional Information

Dietary restriction

A diet stock solution was prepared by mixing 3 parts yeast hydrolysate and 1 part sucrose in distilled water. 40 mg diet per fly per day delivered in 3 mL was found to be ad libitum and was selected as the base diet level (100%). Diet reduction was implemented by feeding adults one of 12 diets proportionally less total nutrient content relative to the base diet level: 100, 95, 90, 85, 80, 75, 70, 65, 60, 50, 40, and 30%.

decreased

Increased longevity was not observed at any level of diet restriction. in both sexes, mortality increased more sharply and the pattern of survival changed abruptly once the diet level fell to 50% or below of ad libitum.

Carey et al.

2002

12882344

Lifespan Observations Database (ID: 4763)

241

OB_004960