Ageing Phenotype Observation—Data Type 1 : OB_007920
Last modified: Dec 09, 2014. Release 1 • Page created: Jun 02, 2024
Summary
Species |
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Drosophila melanogaster |
Gene |
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Gene Symbol |
NCBI Gene ID |
Akh |
38495 |
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Other Ageing Factor |
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Other Ageing Factor Name |
Dietary restriction |
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Description |
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Knockdown of the adipokinetic hormone (Akh) by RNAi (with an RU486-inducible and ubiquitously expressing Actin 5C-GS Gal4 strain) does not by itself affect lifespan, but significantly inhibits DR-dependent yhe increase in lifespan across a range of yeast concentrations in both females and males. While control females and males exhibit a 113%/22% increase in lifespan under DR, upon Akh inhibition there was a significant reduction in lifespan extension with DR (52%/5%). Global Akh knockdown reduces starvation resistance by 24% upon DR, but no significant change upon AL. Also Akh RNAi repressed the DR-dependent increase in cold-stress resistance. Fat body and neuronal-specific inhibition of Akh by using RU486-inducible S(1)106-GS-Gal4 and Elav-GS-Gal4 enhancer traps, respectively, does not reduce lifespan extension upon DR. But, muscle-specific inhibition of Akh using RU486-inducible muscle enhancer trap (Mhc-GS-Gal4) reduces the DR-dependent increase in lifespan. While control exhibit a 47.2% lifespan extension, animals with muscle-specific Akh inhibition fails to result in any increase upon DR (i.e. completely blocked the DR lifespan extension). Muscle-specific Akh inhibition diminishes the increase in triglyceride synthesis and breakdown present normally under DR. A significant reduction in lifespan extension also occurs with a noninducible muscle driver (Mhc-Gal4). Controls on DR exhibit significant higher levels of spontaneous activity compared to Akh RNAi-inhibited animals at all ages. Akh inhibition reduces the protective effect of DR on age-related decline in muscle function/activity [Katewa et al. 2012]. Fat-body specific Akh RNAi results in increased spontaneous activity and a small but significant increase in lifespan upon AL [Katewa et al. 2012]. Overexpression of Akh in a ubiquitousness manner enhances fat metabolism (significant increase in triglyceride synthesis and breakdown under AL), spontaneous activity (148% on AL and 154% on DR), and lifespan on Al (33%). However, despite and increase in movement under DR, lifespan is not increased under a restricted diet [Katewa et al. 2012]. |
PubMed |
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Source |
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GenDR |
Observation Stable ID |
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OB_007920 |
Download |
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XML |
References
no reference information available
Sources
Ageing-related Data Sources
Additional Data Sources